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© 1992 Oxford University Press

RESEARCH-ARTICLE

Genomic organization of a cDNA (QM) demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells and its localization to Xq28

Kiyotoshi Kaneko*, Hisashi Kobayashi, Osamu Onodera, Tadashi Miyatake1 and Shoji Tsuji

Department of Neurology, Brain Research Institute, Niigata University Niigata 951 1Department of Neurology, Tokyo Medical and Dental University Bunkyo-ku, Tokyo 113, Japan

*To whom correspondence should be addressed at: Department of Neurology, Tokyo Medical and Dental University Bunkyo-ku, Tokyo 113, Japan

Received April 29, 1992; Revised August 17, 1992; Accepted August 17, 1992

Using a cosmid clone derived from human Xq28 as a probe which shows cross-species homology, we isolated cDNA clones and the nucleotide sequence analysis of the cDNA revealed that the cDNA is identical to QM cDNA. The QM cDNA has recently been reported as a cDNA with down-regulation in tumorigenic Wilms' tumor microcell hybrid. Comparison of the nucleotide sequences of the cDNA with those of the genomic DNA allowed us to determine the genomic organization of the QM gene. The QM gene consists of at least 7 exons and is located at Xq28. Southern blot analysis of a somatic cell hybrid panel indicates that the QM genes are scattered at least to chromosome 2, 3, 6, 14, 16, and possibly to other chromosomes. Northern blot analysis demonstrated the QM gene is expressed in all the examined adult human tissues as well as cell lines including HeLa cells, fibroblasts, and somatic cell hybrids with increased expression in liver, spleen, testis, and adrenal gland. The results suggest that the QM gene belongs to a new multi-gene family with yet undetermined function.


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