Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dauwerse, J.G.
Right arrow Articles by van Ommen, G.J.B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dauwerse, J.G.
Right arrow Articles by van Ommen, G.J.B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1992 Oxford University Press

OTHER

Multiple colors by fluorescence in situ hybridization using ratio-labelled DNA probes create a molecular karyotype

J.G. Dauwerse*, J. Wiegant1, A.K. Raap1, M.H. Breuning and G.J.B. van Ommen

Department of Human Genetics, Leiden University Wassenaarseweg 72, 2333 AL Leiden, The Netherlands 1Department of Cytochemistry and Cytometry, Leiden University Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

* To whom correspondence should be addressed

Received July 23, 1992; Revised August 28, 1992; Accepted August 28, 1992

Fluorescence in situ hybridization (FISH) is now widely used for the localization of genomic DNA fragments, and the identification of chromosomes by painting. We now show that half of the chromosomal complement can be painted in twelve different colors by using human chromosome specific libraries carrying three distinct labels mixed in multiple ratios. The photographs are in ‘real’ color rather than ‘colorized’. The painting technique described here can be used for the identification of small or complex chromosomal rearrangements and marker chromosomes in humans or in any other species for which well defined chromosome specific libraries exist in a laboratory equipped with a conventional fluorescence microscope. The versatility of this novel cytogenetic technology may well constitute an advancement comparable to the introduction of chromosome banding and high resolution analysis of chromosomes in prometaphase.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
S. L. Erlandsen, E. Jarroll, P. Wallis, and H. van Keulen
Development of Species-specific rDNA Probes for Giardia by Multiple Fluorescent In Situ Hybridization Combined with Immunocytochemical Identification of Cyst Wall Antigens
J. Histochem. Cytochem., August 1, 2005; 53(8): 917 - 927.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Q.-w. Jin, E. Trelles-Sticken, H. Scherthan, and J. Loidl
Yeast Nuclei Display Prominent Centromere Clustering That Is Reduced in Nondividing Cells and in Meiotic Prophase
J. Cell Biol., April 6, 1998; 141(1): 21 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Simon, B. Reipert, M. M. Eibl, and A. Steinkasserer
Detection of Phosphatidylinositol Glycan Class A Gene Transcripts by RT In Situ PCR Hybridization: A Comparative Study Using Fluorescein, Texas Red, and Digoxigenin-11 dUTP for Color Detection
J. Histochem. Cytochem., December 1, 1997; 45(12): 1659 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
C. T. Caskey
Molecular Medicine: A Spin-off From the Helix
JAMA, April 21, 1993; 269(15): 1986 - 1992.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
R. Dirks, F. van de Rijke, S Fujishita, M van der Ploeg, and A. Raap
Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes
J. Cell Sci., January 4, 1993; 104(4): 1187 - 1197.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.