© 1992 Oxford University Press
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A yeast artificial chromosome contig spanning the Charcot — Marie — Tooth disease type 1A duplication region
Department of Neurology, University of Pennsylvania, School of Medicine 422 Curie Boulevard, Philadelphia, PA 19104-6146 1Institute for Molecular Genetics and Human Genome Center, One Baylor Plaza, Baylor College of Medicine Houston, TX 77030, USA 2Department of Human Genetics, State University of Leiden, Sylvius Laboratories Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
* To whom correspondence should be addressed
Received July 20, 1992; Revised September 4, 1992; Accepted September 4, 1992
A contiguous set of 43 overlapping yeast artificial chromosome (YAC) clones has been developed for the Charcot—Marie—Tooth disease type 1A (CMT1A) duplication region of chromosome 17p11.2. The contig spans approximately 2.0 Mb and can be represented in a minimum of five overlapping YACs. The YAC clones were isolated from two total human genomic YAC libraries and from YAC libraries made from rodent-human hybrid cell lines. YAC clones were isolated from the libraries by polymerase chain reaction (PCR) technique. Localization to chromosome 17p11.2 was confirmed by fluorescence in situ hybridization. Overlap between the YAC clones was detected by inter-Alu PCR amplification of the YACs and by cross hybridization of the YACs with YAC insert ends obtained by Vectorette PCR. This YAC contig is a useful resource for analyzing and mapping all the genes contained within the CMT1A duplication.