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© 1992 Oxford University Press

OTHER

Localization of the synovial sarcoma t(X;18)(p11.2;q11.2) breakpoint by fluorescence in situ hybridization

Jennifer C. Knight*, Brian R. Reeves1, Lyndal Kearney2, Anthony P. Monaco3, Hans Lehrach4 and Colin S. Cooper5

Section of Cell Biology and Experimental Pathology, Institute of Cancer Research 15 Cotswold Road, Belmont, Surrey SM2 5NG 1Division of Cell and Molecular Biology, Institute of Child Health 30 Guilford Street, London WC1N 1EH 2Department of Medical Oncology, Imperial Cancer Research Fund, St Bartholomew's Hospital London EC1A 7BE 3Human Genetics Laboratory, Imperial Cancer Research Fund, John Radcliffe Hospital Headington, Oxford OX3 9DU 4Genome Analysis Laboratory, Imperial Cancer Research Fund PO Box 123, London WC2A 3PX 5Section of Molecular Carcinogenesis, Institute of Cancer Research 15 Cotswold Road, Belmont, Surrey SM2 5NG, UK

*To whom correspondence should be addressed at: Soft Tissue Tumour Unit, St Thomas's Hospital, London SEI 7EH, UK

Received April 22, 1992; Revised September 14, 1992; Accepted September 14, 1992

A high proportion of synovial sarcomas contain a chromosome translocation t(X;18)(p11.2;q11.2). We have previously used somatic cell hybrids derived from an established cell line, SS255, to map the X chromosome breakpoint to the interval flanked by the markers DXS14 and DXS146. In this study we have examined these hybrids with thirteen additional markers located at Xp11.3-Xcen, by Southern hybridization. Based on these results we have delimited the breakpoint as follows Xpter-DXS228-(UBE1-OATL1-TIMP-DXS226)-(DXS255-TFE3-ELK1-DXS146)-OATL2-X; 18-(DXS14-DXS422-DXS423-DXS674-DXS679)-Xcen. Confirmation of the breakpoint location has been obtained by analysis of two synovial sarcoma cell lines, SS255 and HA2243, using fluorescence in situ hybridization. A 350kb YAC probe spanning the DXS423 locus hybridized only to the derivative X chromosome, showing that it maps proximal to the breakpoint. Two YAC probes of 300kb and 450kb, containing the OATL2 locus, hybridized to both derivative chromosomes, indicating that these YACs span the translocation breakpoint. Similar results were obtained with both cell lines. The identification of YACs that span the t(X;18) breakpoint now facilitates a strategy for cloning candidate genes from this precisely defined region.


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