Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (67)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Waelter, S.
Right arrow Articles by Wanker, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waelter, S.
Right arrow Articles by Wanker, E. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2001, Vol. 10, No. 17 1807-1817
© 2001 Oxford University Press

The huntingtin interacting protein HIP1 is a clathrin and {alpha}-adaptin-binding protein involved in receptor-mediated endocytosis

Stephanie Waelter, Eberhard Scherzinger, Renate Hasenbank, Eckhard Nordhoff, Rudi Lurz, Heike Goehler, Christine Gauss1, Kirupa Sathasivam2, Gillian P. Bates2, Hans Lehrach and Erich E. Wanker+

1Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin (Dahlem), Germany, 1GPC Biotech AG, Fraunhoferstrasse 20, D-82152 Martinsried, Germany and 2Division of Medical and Molecular Genetics, United Medical and Dental Schools, Guy’s Hospital, London SE1 7EH, UK

The huntingtin interacting protein (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. It is a multidomain protein containing an N-terminal ENTH domain, a central coiled-coil forming region and a C-terminal actin-binding domain. In the present study we have identified three HIP1 associated proteins, clathrin heavy chain and {alpha}-adaptin A and C. In vitro binding studies revealed that the central coiled-coil domain is required for the interaction of HIP1 with clathrin, whereas DPF-like motifs located upstream to this domain are important for the binding of HIP1 to the C-terminal ‘appendage’ domain of {alpha}-adaptin A and C. Expression of full length HIP1 in mammalian cells resulted in a punctate cytoplasmic immunostaining characteristic of clathrin-coated vesicles. In contrast, when a truncated HIP1 protein containing both the DPF-like motifs and the coiled-coil domain was overexpressed, large perinuclear vesicle-like structures containing HIP1, huntingtin, clathrin and endocytosed transferrin were observed, indicating that HIP1 is an endocytic protein, the structural integrity of which is crucial for maintenance of normal vesicle size in vivo.

+ To whom correspondence should be addressed. Tel: + 49 30 8413 1351; Fax: +49 30 8413 1380; Email: wanker@molgen.mpg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
C. Zabel, L. Mao, B. Woodman, M. Rohe, M. A. Wacker, Y. Klare, A. Koppelstatter, G. Nebrich, O. Klein, S. Grams, et al.
A Large Number of Protein Expression Changes Occur Early in Life and Precede Phenotype Onset in a Mouse Model for Huntington Disease
Mol. Cell. Proteomics, April 1, 2009; 8(4): 720 - 734.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. W. Graves, S. T. Philips, S. V. Bradley, K. I. Oravecz-Wilson, L. Li, A. Gauvin, and T. S. Ross
Use of a Cryptic Splice Site for the Expression of Huntingtin Interacting Protein 1 in Select Normal and Neoplastic Tissues
Cancer Res., February 15, 2008; 68(4): 1064 - 1073.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Poupon, M. Girard, V. Legendre-Guillemin, S. Thomas, L. Bourbonniere, J. Philie, N. A. Bright, and P. S. McPherson
Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly
PNAS, January 8, 2008; 105(1): 168 - 173.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. J. Milnerwood and L. A. Raymond
Corticostriatal synaptic function in mouse models of Huntington's disease: early effects of huntingtin repeat length and protein load
J. Physiol., December 15, 2007; 585(3): 817 - 831.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. A. Parker, M. Metzler, J. Georgiou, M. Mage, J. C. Roder, A. M. Rose, M. R. Hayden, and C. Neri
Huntingtin-Interacting Protein 1 Influences Worm and Mouse Presynaptic Function and Protects Caenorhabditis elegans Neurons against Mutant Polyglutamine Toxicity
J. Neurosci., October 10, 2007; 27(41): 11056 - 11064.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. V. Bradley, M. R. Smith, T. S. Hyun, P. C. Lucas, L. Li, D. Antonuk, I. Joshi, F. Jin, and T. S. Ross
Aberrant Huntingtin Interacting Protein 1 in Lymphoid Malignancies
Cancer Res., September 15, 2007; 67(18): 8923 - 8931.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. V. Bradley, T. S. Hyun, K. I. Oravecz-Wilson, L. Li, E. I. Waldorff, A. N. Ermilov, S. A. Goldstein, C. X. Zhang, D. G. Drubin, K. Varela, et al.
Degenerative phenotypes caused by the combined deficiency of murine HIP1 and HIP1r are rescued by human HIP1
Hum. Mol. Genet., June 1, 2007; 16(11): 1279 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. V. Bradley, E. C. Holland, G. Y. Liu, D. Thomas, T. S. Hyun, and T. S. Ross
Huntingtin Interacting Protein 1 Is a Novel Brain Tumor Marker that Associates with Epidermal Growth Factor Receptor
Cancer Res., April 15, 2007; 67(8): 3609 - 3615.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Metzler, L. Gan, T. Pan Wong, L. Liu, J. Helm, L. Liu, J. Georgiou, Y. Wang, N. Bissada, K. Cheng, et al.
NMDA Receptor Function and NMDA Receptor-Dependent Phosphorylation of Huntingtin Is Altered by the Endocytic Protein HIP1
J. Neurosci., February 28, 2007; 27(9): 2298 - 2308.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Tagawa, S. Marubuchi, M.-L. Qi, Y. Enokido, T. Tamura, R. Inagaki, M. Murata, I. Kanazawa, E. E. Wanker, and H. Okazawa
The Induction Levels of Heat Shock Protein 70 Differentiate the Vulnerabilities to Mutant Huntingtin among Neuronal Subtypes
J. Neurosci., January 24, 2007; 27(4): 868 - 880.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
E. Trushina, R. D. Singh, R. B. Dyer, S. Cao, V. H. Shah, R. G. Parton, R. E. Pagano, and C. T. McMurray
Mutant huntingtin inhibits clathrin-independent endocytosis and causes accumulation of cholesterol in vitro and in vivo
Hum. Mol. Genet., December 15, 2006; 15(24): 3578 - 3591.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. B. Kegel, E. Sapp, J. Yoder, B. Cuiffo, L. Sobin, Y. J. Kim, Z.-H. Qin, M. R. Hayden, N. Aronin, D. L. Scott, et al.
Huntingtin Associates with Acidic Phospholipids at the Plasma Membrane
J. Biol. Chem., October 28, 2005; 280(43): 36464 - 36473.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
I. G. Mills, L. Gaughan, C. Robson, T. Ross, S. McCracken, J. Kelly, and D. E. Neal
Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors
J. Cell Biol., July 18, 2005; 170(2): 191 - 200.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Legendre-Guillemin, M. Metzler, J.-F. Lemaire, J. Philie, L. Gan, M. R. Hayden, and P. S. McPherson
Huntingtin Interacting Protein 1 (HIP1) Regulates Clathrin Assembly through Direct Binding to the Regulatory Region of the Clathrin Light Chain
J. Biol. Chem., February 18, 2005; 280(7): 6101 - 6108.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-Y. Chen and F. M. Brodsky
Huntingtin-interacting Protein 1 (Hip1) and Hip1-related Protein (Hip1R) Bind the Conserved Sequence of Clathrin Light Chains and Thereby Influence Clathrin Assembly in Vitro and Actin Distribution in Vivo
J. Biol. Chem., February 18, 2005; 280(7): 6109 - 6117.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. T. Kittler, P. Thomas, V. Tretter, Y. D. Bogdanov, V. Haucke, T. G. Smart, and S. J. Moss
Huntingtin-associated protein 1 regulates inhibitory synaptic transmission by modulating {gamma}-aminobutyric acid type A receptor membrane trafficking
PNAS, August 24, 2004; 101(34): 12736 - 12741.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
G. Schilling, A. V. Savonenko, A. Klevytska, J. L. Morton, S. M. Tucker, M. Poirier, A. Gale, N. Chan, V. Gonzales, H. H. Slunt, et al.
Nuclear-targeting of mutant huntingtin fragments produces Huntington's disease-like phenotypes in transgenic mice
Hum. Mol. Genet., August 1, 2004; 13(15): 1599 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. G. Roth
Phosphoinositides in Constitutive Membrane Traffic
Physiol Rev, July 1, 2004; 84(3): 699 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. S. Hyun, L. Li, K. I. Oravecz-Wilson, S. V. Bradley, M. M. Provot, A. J. Munaco, I. F. Mizukami, H. Sun, and T. S. Ross
Hip1-related Mutant Mice Grow and Develop Normally but Have Accelerated Spinal Abnormalities and Dwarfism in the Absence of HIP1
Mol. Cell. Biol., May 15, 2004; 24(10): 4329 - 4340.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
K. I. Oravecz-Wilson, M. J. Kiel, L. Li, D. S. Rao, D. Saint-Dic, P. D. Kumar, M. M. Provot, K. D. Hankenson, V. N. Reddy, A. P. Lieberman, et al.
Huntingtin Interacting Protein 1 mutations lead to abnormal hematopoiesis, spinal defects and cataracts
Hum. Mol. Genet., April 15, 2004; 13(8): 851 - 867.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. S. Hyun, D. S. Rao, D. Saint-Dic, L. E. Michael, P. D. Kumar, S. V. Bradley, I. F. Mizukami, K. I. Oravecz-Wilson, and T. S. Ross
HIP1 and HIP1r Stabilize Receptor Tyrosine Kinases and Bind 3-Phosphoinositides via Epsin N-terminal Homology Domains
J. Biol. Chem., April 2, 2004; 279(14): 14294 - 14306.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. E. Y. Engqvist-Goldstein, C. X. Zhang, S. Carreno, C. Barroso, J. E. Heuser, and D. G. Drubin
RNAi-mediated Hip1R Silencing Results in Stable Association between the Endocytic Machinery and the Actin Assembly Machinery
Mol. Biol. Cell, April 1, 2004; 15(4): 1666 - 1679.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
L. Basel-Vanagaite, R. Straussberg, H. Ovadia, A. Kaplan, N. Magal, Z. Shorer, H. Shalev, C. Walsh, and M. Shohat
Infantile bilateral striatal necrosis maps to chromosome 19q
Neurology, January 13, 2004; 62(1): 87 - 90.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z.-H. Qin, Y. Wang, E. Sapp, B. Cuiffo, E. Wanker, M. R. Hayden, K. B. Kegel, N. Aronin, and M. DiFiglia
Huntingtin Bodies Sequester Vesicle-Associated Proteins by a Polyproline-Dependent Interaction
J. Neurosci., January 7, 2004; 24(1): 269 - 281.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Legendre-Guillemin, S. Wasiak, N. K. Hussain, A. Angers, and P. S. McPherson
ENTH/ANTH proteins and clathrin-mediated membrane budding
J. Cell Sci., January 1, 2004; 117(1): 9 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Li, T. Wyman, Z.-X. Yu, S.-H. Li, and X.-J. Li
Abnormal association of mutant huntingtin with synaptic vesicles inhibits glutamate release
Hum. Mol. Genet., August 15, 2003; 12(16): 2021 - 2030.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. Gines, I. S. Seong, E. Fossale, E. Ivanova, F. Trettel, J. F. Gusella, V. C. Wheeler, F. Persichetti, and M. E. MacDonald
Specific progressive cAMP reduction implicates energy deficit in presymptomatic Huntington's disease knock-in mice
Hum. Mol. Genet., March 1, 2003; 12(5): 497 - 508.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. D. Finley, P. T. Edeen, R. C. Cumming, M. D. Mardahl-Dumesnil, B. J. Taylor, M. H. Rodriguez, C. E. Hwang, M. Benedetti, and M. McKeown
blue cheese Mutations Define a Novel, Conserved Gene Involved in Progressive Neural Degeneration
J. Neurosci., February 15, 2003; 23(4): 1254 - 1264.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
R. R. Singaraja, S. Hadano, M. Metzler, S. Givan, C. L. Wellington, S. Warby, A. Yanai, C.-A. Gutekunst, B. R. Leavitt, H. Yi, et al.
HIP14, a novel ankyrin domain-containing protein, links huntingtin to intracellular trafficking and endocytosis
Hum. Mol. Genet., November 1, 2002; 11(23): 2815 - 2828.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Kalthoff, S. Groos, R. Kohl, S. Mahrhold, and E. J. Ungewickell
Clint: A Novel Clathrin-binding ENTH-Domain Protein at the Golgi
Mol. Biol. Cell, November 1, 2002; 13(11): 4060 - 4073.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Modregger, N. A. DiProspero, V. Charles, D. A. Tagle, and M. Plomann
PACSIN 1 interacts with huntingtin and is absent from synaptic varicosities in presymptomatic Huntington's disease brains
Hum. Mol. Genet., October 2, 2002; 11(21): 2547 - 2558.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. R. Henry, K. D'Hondt, J. Chang, T. Newpher, K. Huang, R. T. Hudson, H. Riezman, and S. K. Lemmon
Scd5p and Clathrin Function Are Important for Cortical Actin Organization, Endocytosis, and Localization of Sla2p in Yeast
Mol. Biol. Cell, August 1, 2002; 13(8): 2607 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Li, L.-S. Chin, A. I. Levey, and L. Li
Huntingtin-associated Protein 1 Interacts with Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate and Functions in Endosomal Trafficking
J. Biol. Chem., July 26, 2002; 277(31): 28212 - 28221.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Legendre-Guillemin, M. Metzler, M. Charbonneau, L. Gan, V. Chopra, J. Philie, M. R. Hayden, and P. S. McPherson
HIP1 and HIP12 Display Differential Binding to F-actin, AP2, and Clathrin. IDENTIFICATION OF A NOVEL INTERACTION WITH CLATHRIN LIGHT CHAIN
J. Biol. Chem., May 24, 2002; 277(22): 19897 - 19904.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.