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Human Molecular Genetics, 2001, Vol. 10, No. 5 477-484
© 2001 Oxford University Press

WFS1 (Wolfram syndrome 1) gene product: predominant subcellular localization to endoplasmic reticulum in cultured cells and neuronal expression in rat brain

Koumei Takeda1,+, Hiroshi Inoue+,1, Yukio Tanizawa1, Yumiko Matsuzaki2, Jun Oba2, Yoshifumi Watanabe3, Koh Shinoda2 and Yoshitomo Oka1,§

1Third Department of Internal Medicine, 2Department of Anatomy, and 3Department of Neuropsychiatry, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan

Wolfram (DIDMOAD) syndrome is an autosomal recessive neurodegenerative disorder accompanied by insulin-dependent diabetes mellitus and progressive optic atrophy. Recent positional cloning led to identification of the WFS1 (Wolfram syndrome 1) gene, a member of a novel gene family of unknown function. In this study, we generated a specific antibody against the C-terminus of the WFS1 protein and investigated its subcellular localization in cultured cells. We also studied its distribution in the rat brain. Biochemical studies indicated the WFS1 protein to be an integral, endoglycosidase H-sensitive membrane glycoprotein that localizes primarily in the endoplasmic reticulum (ER). Consistent with this, immunofluorescence cell staining of overexpressed WFS1 showed a characteristic reticular pattern over the cytoplasm and overlapped with the ER marker staining. No co-localization of WFS1 with mitochondria argues against an earlier clinical hypothesis that Wolfram syndrome is a mitochondria-mediated disorder. In the rat brain, at both the protein and mRNA level, WFS1 was found to be present predominantly in selected neurons in the hippocampus CA1, amygdaloid areas, olfactory tubercle and superficial layer of the allocortex. These expression sites, i.e. components of the limbic system or structures closely associated with this system, may be involved in the psychiatric, behavioral and emotional abnormalities characteristic of this syndrome. ER localization of WFS1 suggests that this protein plays an as yet undefined role in membrane trafficking, protein processing and/or regulation of ER calcium homeostasis. These studies represent a first step toward the characterization of WFS1 protein, which presumably functions to maintain certain populations of neuronal and endocrine cells.

+ These authors contributed equally to this work

§ To whom correspondence should be addressed. Tel: +81 836 22 2250; Fax: +81 836 22 2256; Email: oka-y@po.cc.yamaguchi-u.ac.jp


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