Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Coleman, S. L.
Right arrow Articles by O'Donovan, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coleman, S. L.
Right arrow Articles by O'Donovan, M. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2002, Vol. 11, No. 16 1817-1821
© 2002 Oxford University Press

Experimental analysis of the annotation of promoters in the public database

Sharon L. Coleman, Paul R. Buckland, Bastiaan Hoogendoorn, Carol Guy, Kaye Smith and Michael C. O'Donovan*

Department of Psychological Medicine, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, UK

Received March 3, 2002; Accepted May 31, 2002

The ability to identify and examine promoter elements is important to researchers who wish to understand how gene expression is regulated in normal and pathological states. Unfortunately, the number of human promoters that have been directly experimentally defined is small. In order to determine if promoter sequences can be identified by simply aligning mRNA and genomic sequences, we have used a reporter gene assay to assess the promoter activity of the immediate 5' region flanking 38 mRNAs mapping to chromosome 21. For comparison, we have measured the activities of 19 sequences not thought to be promoters and 39 sequences taken from the Eukaryotic Promoter Database. Our results suggest that alignment of reference mRNAs to genomic sequence allows promoters to be identified for at least 75% of genes. These data provide the first empirical evidence that the current state of annotation of the genome is sufficient to allow molecular geneticists to correctly identify promoter sequences for most genes for which reference mRNA and genomic sequences are available.

* To whom correspondence should be addressed. Tel: +44 2920743242; Fax: +44 2920747839; Email: odonovanmc{at}cardiff.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
G. Argyropoulos, A. M. Stutz, O. Ilnytska, T. Rice, M. Teran-Garcia, D. C. Rao, C. Bouchard, and T. Rankinen
KIF5B gene sequence variation and response of cardiac stroke volume to regular exercise
Physiol Genomics, January 8, 2009; 36(2): 79 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. Ping, H. Wang, M. Huang, and Z.-s. Liu
Genetic Analysis of Glutathione S-transferase A1 Polymorphism in the Chinese Population and the Influence of Genotype on Enzymatic Properties
Toxicol. Sci., February 1, 2006; 89(2): 438 - 443.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. PsychiatryHome page
P. R. Buckland, B. Hoogendoorn, C. A. Guy, S. K. Smith, S. L. Coleman, and M. C. O'Donovan
Low Gene Expression Conferred by Association of an Allele of the 5-HT2C Receptor Gene With Antipsychotic-Induced Weight Gain
Am J Psychiatry, March 1, 2005; 162(3): 613 - 615.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. R. Buckland
Allele-specific gene expression differences in humans
Hum. Mol. Genet., October 1, 2004; 13(suppl_2): R255 - R260.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Chong, G. Zhang, and V. B. Bajic
FIE2: a program for the extraction of genomic DNA sequences around the start and translation initiation site of human genes
Nucleic Acids Res., July 1, 2003; 31(13): 3546 - 3553.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Bruch, W. A. Schulz, J. Häussler, I. Melzner, S. Brüderlein, P. Möller, R. Kemmerling, W. Vogel, and H. Hameister
Delineation of the 6p22 Amplification Unit in Urinary Bladder Carcinoma Cell Lines
Cancer Res., August 1, 2000; 60(16): 4526 - 4530.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.