Skip Navigation


Human Molecular Genetics Advance Access originally published online on October 7, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
12/23/3087    most recent
ddg335v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mazroui, R.
Right arrow Articles by Khandjian, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mazroui, R.
Right arrow Articles by Khandjian, E. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2003, Vol. 12, No. 23 3087-3096
DOI: 10.1093/hmg/ddg335
© 2003 Oxford University Press

Fragile X Mental Retardation protein determinants required for its association with polyribosomal mRNPs

Rachid Mazroui{dagger}, Marc-Etienne Huot, Sandra Tremblay, Nathalie Boilard, Yves Labelle and Edouard W. Khandjian*

Unité de Recherche en Génétique Humaine et Moléculaire, Centre de Recherche Hôpital Saint-François d'Assise, Le CHUQ, Québec, (Qc) G1L 3L5 and Département de Biologie Médicale, Faculté de Médecine, Université Laval, Québec, Canada

Received July 17, 2003; Accepted September 29, 2003

Fragile X Mental Retardation protein (FMRP) is an RNA-binding protein that contains multiple domains with apparently differential affinity to mRNA and to the ribonucleotide homopolymer poly(G). Attempts have been made to map the RNA-binding sites along the protein sequence with a view to determining which of the KH1, KH2 and RGG domains are required to recognize and bind to RNA. While these studies have greatly contributed to the delineation of domains that bind homopolymers or mRNA in vitro, little is known concerning their implications in FMRP function(s) in vivo. To address this question, we have prepared a series of FMRP versions, in which each known in vitro functional domain has been individually deleted, leaving the rest of the protein intact. Constructs with deletions in the protein–protein interaction and RNA-binding as well as in the phosphorylation domains were expressed in STEK-KO cells lacking FMRP and their recruitment into polyribosomal mRNPs and their intra-cellular localization were determined. Our results indicate that the KH RNA-binding domains and the Protein–Protein Interacting domain are essential for FMRP to associate with polyribosomal mRNPs, while the RGG box and the phosphorylated domains are dispensable.

* To whom correspondence should be addressed at: URGHM, Centre de Recherche Hôpital St-François d'Assise, 10 rue de l'Espinay, Québec G1L 3L5, PQ, Canada. Tel: +1 4185254402; Fax: +1 4185254195; Email: edward.khandjian{at}crsfa.ulaval.ca

{dagger} Present address: Department of Biochemistry, McGill University, Montréal, PQ, Canada.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Piazzon, F. Rage, F. Schlotter, H. Moine, C. Branlant, and S. Massenet
In Vitro and in Cellulo Evidences for Association of the Survival of Motor Neuron Complex with the Fragile X Mental Retardation Protein
J. Biol. Chem., February 29, 2008; 283(9): 5598 - 5610.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. L. Hayashi, B. S. S. Rao, J.-S. Seo, H.-S. Choi, B. M. Dolan, S.-Y. Choi, S. Chattarji, and S. Tonegawa
Inhibition of p21-activated kinase rescues symptoms of fragile X syndrome in mice
PNAS, July 3, 2007; 104(27): 11489 - 11494.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Mazroui, S. Di Marco, R. J. Kaufman, and I.-E. Gallouzi
Inhibition of the Ubiquitin-Proteasome System Induces Stress Granule Formation
Mol. Biol. Cell, July 1, 2007; 18(7): 2603 - 2618.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. Banerjee, S. Nayar, S. Hebbar, C. F. Fox, M. C. Jacobs, J. H. Park, J. J. Fernandes, and T. C. Dockendorff
Substitution of Critical Isoleucines in the KH Domains of Drosophila Fragile X Protein Results in Partial Loss-of-Function Phenotypes
Genetics, March 1, 2007; 175(3): 1241 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Bechara, L. Davidovic, M. Melko, M. Bensaid, S. Tremblay, J. Grosgeorge, E. W. Khandjian, E. Lalli, and B. Bardoni
Fragile X related protein 1 isoforms differentially modulate the affinity of fragile X mental retardation protein for G-quartet RNA structure
Nucleic Acids Res., January 12, 2007; 35(1): 299 - 306.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Dolzhanskaya, G. Merz, J. M. Aletta, and R. B. Denman
Methylation regulates the intracellular protein-protein and protein-RNA interactions of FMRP.
J. Cell Sci., May 1, 2006; 119(Pt 9): 1933 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
L. Davidovic, E. Bechara, M. Gravel, X. H. Jaglin, S. Tremblay, A. Sik, B. Bardoni, and E. W. Khandjian
The nuclear MicroSpherule protein 58 is a novel RNA-binding protein that interacts with fragile X mental retardation protein in polyribosomal mRNPs from neurons
Hum. Mol. Genet., May 1, 2006; 15(9): 1525 - 1538.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. C. Darnell, C. E. Fraser, O. Mostovetsky, G. Stefani, T. A. Jones, S. R. Eddy, and R. B. Darnell
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes
Genes & Dev., April 15, 2005; 19(8): 903 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. W. Khandjian, M.-E. Huot, S. Tremblay, L. Davidovic, R. Mazroui, and B. Bardoni
Biochemical evidence for the association of fragile X mental retardation protein with brain polyribosomal ribonucleoparticles
PNAS, September 7, 2004; 101(36): 13357 - 13362.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Gabus, R. Mazroui, S. Tremblay, E. W. Khandjian, and J.-L. Darlix
The fragile X mental retardation protein has nucleic acid chaperone properties
Nucleic Acids Res., April 19, 2004; 32(7): 2129 - 2137.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.