Skip Navigation


Human Molecular Genetics Advance Access originally published online on September 30, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
12/23/3109    most recent
ddg330v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Craig, J. M.
Right arrow Articles by Choo, K.H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Craig, J. M.
Right arrow Articles by Choo, K.H. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2003, Vol. 12, No. 23 3109-3121
DOI: 10.1093/hmg/ddg330
© 2003 Oxford University Press

Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns

Jeffrey M. Craig, Elizabeth Earle, Paul Canham, Lee H. Wong, Melissa Anderson and K.H. Andy Choo*

Murdoch Childrens Research Institute, Royal Children's Hospital, Flemington Road, Melbourne, Victoria 3052, Australia

Received June 26, 2003; Revised September 15, 2003; Accepted September 23, 2003

We have examined the metaphase chromosomal localization of 15 proteins that have previously been described as involved in mammalian chromatin modification and/or transcriptional modulation. Immunofluorescence data indicate that all the proteins localize to human and mouse centromeres, a neocentromere, and the active centromere of a dicentric chromosome, with six of these proteins (Sin3A, PCAF, MYST, MBD2, ORC2, P300/CBP) being demonstrated at mammalian centromeres for the first time. Most of these proteins fall into two distinct chromosomal distribution patterns: (a) kinetochore-associated proteins (Sin3A, PCAF, MYST and BAF180), which colocalize with metaphase kinetochores, but not any of the pericentric and other major heterochromatic regions; and (b) heterochromatin-associated proteins (MeCP2, MBD1, MBD2, ATRX, HP1{alpha}, HDAC1, HDAC2, DNMT1 and DNMT3b), which colocalize with centromeric/pericentric heterochromatin and all other major heterochromatic sites. A heterogeneous third group (c) consists of the origin recognition complex subunit ORC2 and the histone acetyltransferase P300/CBP, which associate generally with kinetochores in humans and centromeric/pericentric heterochromatin in mouse, with some minor differences in localization. These observations indicate an extensive sharing of protein components involved in chromatin modification at gene loci, centromeres and various chromosomal heterochromatic landmarks. The definition of distinct patterns of chromosomal distribution for these proteins provides a useful basis for the further investigation of the broad-ranging roles of these proteins.

* To whom correspondence should be addressed. Tel: +61 383416306; Fax: +61 393481391; Email: choo{at}cryptic.rch.unimelb.edu.au


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
K. Ishii, Y. Ogiyama, Y. Chikashige, S. Soejima, F. Masuda, T. Kakuma, Y. Hiraoka, and K. Takahashi
Heterochromatin Integrity Affects Chromosome Reorganization After Centromere Dysfunction
Science, August 22, 2008; 321(5892): 1088 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Bouzinba-Segard, A. Guais, and C. Francastel
Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function
PNAS, June 6, 2006; 103(23): 8709 - 8714.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. K. Sjoberg, E. Shestakova, Z. Mansuroglu, R. B. Maccioni, and E. Bonnefoy
Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization
J. Cell Sci., May 15, 2006; 119(10): 2025 - 2034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Vigodner, T. Ishikawa, P. N. Schlegel, and P. L. Morris
SUMO-1, human male germ cell development, and the androgen receptor in the testis of men with normal and abnormal spermatogenesis
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E1022 - E1033.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Tamada, N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama, and N. Kikyo
Chromatin Decondensation and Nuclear Reprogramming by Nucleoplasmin
Mol. Cell. Biol., February 15, 2006; 26(4): 1259 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. C. Chueh, L. H. Wong, N. Wong, and K.H. A. Choo
Variable and hierarchical size distribution of L1-retroelement-enriched CENP-A clusters within a functional human neocentromere
Hum. Mol. Genet., January 1, 2005; 14(1): 85 - 93.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L.-Y. Wong, G. A. Matchett, and A. C. Wilson
Transcriptional Activation by the Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Is Facilitated by an N-Terminal Chromatin-Binding Motif
J. Virol., September 15, 2004; 78(18): 10074 - 10085.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. Guenatri, D. Bailly, C. Maison, and G. Almouzni
Mouse centric and pericentric satellite repeats form distinct functional heterochromatin
J. Cell Biol., August 16, 2004; 166(4): 493 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. M. Geiman, U. T. Sankpal, A. K. Robertson, Y. Chen, M. Mazumdar, J. T. Heale, J. A. Schmiesing, W. Kim, K. Yokomori, Y. Zhao, et al.
Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery
Nucleic Acids Res., May 17, 2004; 32(9): 2716 - 2729.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. J. Amor, K. Bentley, J. Ryan, J. Perry, L. Wong, H. Slater, and K. H. A. Choo
Human centromere repositioning "in progress"
PNAS, April 27, 2004; 101(17): 6542 - 6547.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.