Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Furey, T. S.
Right arrow Articles by Haussler, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furey, T. S.
Right arrow Articles by Haussler, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2003, Vol. 12, No. 9 1037-1044
DOI: 10.1093/hmg/ddg113
© 2003 Oxford University Press

Integration of the cytogenetic map with the draft human genome sequence

Terrence S. Furey* and David Haussler

Howard Hughes Medical Institute, Department of Computer Science, 321 Baskin Engineering Bldg, University of California, Santa Cruz, CA 95064, USA

Received January 7, 2003; Accepted February 25, 2003

Chemically staining metaphase chromosomes resulting in an alternating dark and light banding pattern provide a tool by which abnormalities in chromosomes from diseased cells can be identified. The localization of these aberrations to a chromosomal region provides clues as to which gene or genes may contribute to a particular disease. With the sequencing of the human genome, it became critical to determine the positions of these cytogenetic bands within the sequence in order to take advantage of vast amount of information now anchored to the sequence, especially the locations of genes. The molecular basis of cytogenetic bands is not well understood, therefore their positions cannot be determined solely based on sequence information. We developed a dynamic programming algorithm that employs results from ~9500 fluorescence in situ hybridization experiments to approximate the locations of the 850 high-resolution bands in the June 2002 version of the draft human genome sequence. These band predictions support previously identified correlations between band stain intensity and certain structural characteristics of chromosomes, namely GC content, repeat structure content, CpG island density, gene density and degree of condensation.

* To whom correspondence should be addressed. Tel: +1 8314595431; Fax: +1 8314594829; Email: booch{at}cse.ucsc.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BioinformaticsHome page
K.-H. Yen, C.-L. Ho, and C. Lee
The analysis of inconsistencies between cytogenetic annotations and sequence mapping by defining the imprecision zones of cytogenetic banding
Bioinformatics, April 1, 2009; 25(7): 845 - 852.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
I. Kraus, C. Driesch, S. Vinokurova, E. Hovig, A. Schneider, M. von Knebel Doeberitz, and M. Durst
The Majority of Viral-Cellular Fusion Transcripts in Cervical Carcinomas Cotranscribe Cellular Sequences of Known or Predicted Genes
Cancer Res., April 1, 2008; 68(7): 2514 - 2522.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. R. Brown, C. J. Kennedy, V. A. Delmar, D. J. Forbes, and P. A. Silver
Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes
Genes & Dev., March 1, 2008; 22(5): 627 - 639.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
R. Thomas, S. E. Duke, S. K. Bloom, T. E. Breen, A. C. Young, E. Feiste, E. L. Seiser, P.-C. Tsai, C. F. Langford, P. Ellis, et al.
A Cytogenetically Characterized, Genome-Anchored 10-Mb BAC Set and CGH Array for the Domestic Dog
J. Hered., August 16, 2007; (2007) esm053v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Goetze, J. Mateos-Langerak, H. J. Gierman, W. de Leeuw, O. Giromus, M. H. G. Indemans, J. Koster, V. Ondrej, R. Versteeg, and R. van Driel
The Three-Dimensional Structure of Human Interphase Chromosomes Is Related to the Transcriptome Map
Mol. Cell. Biol., June 15, 2007; 27(12): 4475 - 4487.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Shimizu and K. Shingaki
Macroscopic folding and replication of the homogeneously staining region in late S phase leads to the appearance of replication bands in mitotic chromosomes
J. Cell Sci., October 15, 2004; 117(22): 5303 - 5312.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Chen, M. Sun, W. J. Kent, X. Huang, H. Xie, W. Wang, G. Zhou, R. Z. Shi, and J. D. Rowley
Over 20% of human transcripts might form sense-antisense pairs
Nucleic Acids Res., September 8, 2004; 32(16): 4812 - 4820.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. I. Jensen-Seaman, T. S. Furey, B. A. Payseur, Y. Lu, K. M. Roskin, C.-F. Chen, M. A. Thomas, D. Haussler, and H. J. Jacob
Comparative Recombination Rates in the Rat, Mouse, and Human Genomes
Genome Res., April 1, 2004; 14(4): 528 - 538.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.