Skip Navigation


Human Molecular Genetics Advance Access originally published online on March 17, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
13/10/1041    most recent
ddh118v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Goodier, J. L.
Right arrow Articles by Kazazian, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goodier, J. L.
Right arrow Articles by Kazazian, H. H., Jr
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2004, Vol. 13, No. 10 1041-1048
DOI: 10.1093/hmg/ddh118
Human Molecular Genetics, Vol. 13, No. 10 © Oxford University Press 2004; all rights reserved

A potential role for the nucleolus in L1 retrotransposition

John L. Goodier1,*, Eric M. Ostertag1,2, Kurt A. Engleka1, Maria C. Seleme1 and Haig H. Kazazian, Jr1

1Department of Genetics, University of Pennsylvania School of Medicine and 2Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA, USA

Received January 13, 2004; Accepted March 9, 2004

Determining the subcellular localization of the L1 ORF2 protein (ORF2p) has been impossible to date because of technical limitations in detecting either endogenous or overexpressed forms of the protein. Here we report visualization of the full-length ORF2p in cultured human cells following expression in a modified vaccinia virus/T7 RNA polymerase (MVA/T7RP) system. The MVA/T7RP system was used to ascertain subcellular localization of L1 ORF1p and ORF2p both as fusions with green fluorescent protein and by immunocytochemistry. Full-length ORF2p was predominantly cytoplasmic, while carboxy-terminal-deleted ORF2p localized additionally to the nucleolus. We mapped a functional nucleolar localization signal in ORF2p. ORF1p appeared in the cytoplasm with a speckled pattern and colocalized with ORF2p in nucleoli in a subset of cells. These findings help explain the presence of chimeras between L1s and small RNA gene sequences recently discovered in the human genome.

* To whom correspondence should be addressed at: Department of Genetics, University of Pennsylvania School of Medicine, Room 515 CRB, 415 Curie Blvd, Philadelphia, PA 19104, USA. Tel: +1 2158982080; Fax: +1 2155737760; Email: jgoodier{at}mail.med.upenn.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome Res.Home page
V. P. Belancio, D. J. Hedges, and P. Deininger
Mammalian non-LTR retrotransposons: For better or worse, in sickness and in health
Genome Res., March 1, 2008; 18(3): 343 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kirilyuk, G. V. Tolstonog, A. Damert, U. Held, S. Hahn, R. Lower, C. Buschmann, A. V. Horn, P. Traub, and G. G. Schumann
Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells
Nucleic Acids Res., February 2, 2008; 36(2): 648 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. L. Goodier, L. Zhang, M. R. Vetter, and H. H. Kazazian Jr.
LINE-1 ORF1 Protein Localizes in Stress Granules with Other RNA-Binding Proteins, Including Components of RNA Interference RNA-Induced Silencing Complex
Mol. Cell. Biol., September 15, 2007; 27(18): 6469 - 6483.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Kinomoto, T. Kanno, M. Shimura, Y. Ishizaka, A. Kojima, T. Kurata, T. Sata, and K. Tokunaga
All APOBEC3 family proteins differentially inhibit LINE-1 retrotransposition
Nucleic Acids Res., May 14, 2007; 35(9): 2955 - 2964.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Matsumoto, M. Hamada, M. Osanai, and H. Fujiwara
Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1.
Mol. Cell. Biol., July 1, 2006; 26(13): 5168 - 5179.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Kubo, M. d. C. Seleme, H. S. Soifer, J. L. G. Perez, J. V. Moran, H. H. Kazazian Jr., and N. Kasahara
L1 retrotransposition in nondividing and primary human somatic cells
PNAS, May 23, 2006; 103(21): 8036 - 8041.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
E. Lucchinetti, J. Feng, R. d. Silva, G. V. Tolstonog, M. C. Schaub, G. G. Schumann, and M. Zaugg
Inhibition of LINE-1 expression in the heart decreases ischemic damage by activation of Akt/PKB signaling
Physiol Genomics, April 13, 2006; 25(2): 314 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. W.-L. Li, J. Li, S. L. Timmerman, L. A. Krushel, and S. L. Martin
The dicistronic RNA from the mouse LINE-1 retrotransposon contains an internal ribosome entry site upstream of each ORF: implications for retrotransposition
Nucleic Acids Res., February 6, 2006; 34(3): 853 - 864.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. S. Alisch, J. L. Garcia-Perez, A. R. Muotri, F. H. Gage, and J. V. Moran
Unconventional translation of mammalian LINE-1 retrotransposons
Genes & Dev., January 15, 2006; 20(2): 210 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Gilbert, S. Lutz, T. A. Morrish, and J. V. Moran
Multiple Fates of L1 Retrotransposition Intermediates in Cultured Human Cells
Mol. Cell. Biol., September 1, 2005; 25(17): 7780 - 7795.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.