Skip Navigation


Human Molecular Genetics Advance Access originally published online on June 30, 2004
Human Molecular Genetics 2004 13(17):1919-1932; doi:10.1093/hmg/ddh193
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
13/17/1919    most recent
ddh193v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lillard-Wetherell, K.
Right arrow Articles by Groden, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lillard-Wetherell, K.
Right arrow Articles by Groden, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, Vol. 13, No. 17 © Oxford University Press 2004; all rights reserved

Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2

Kate Lillard-Wetherell1, Amrita Machwe2, Gregory T. Langland1, Kelly A. Combs1, Gregory K. Behbehani1, Steven A. Schonberg3,4, James German5, John J. Turchi6, David K. Orren2 and Joanna Groden1,*

1Department of Molecular Genetics, Biochemistry and Microbiology, Howard Hughes Medical Institute, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA, 2Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA, 3Department of Pediatrics, George Washington University School of Medicine, Washington DC 20052, USA, 4American Quest Laboratories, Inc., Chantilly, VA 20153, USA, 5Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA and 6Department of Biochemistry and Molecular Biology, Wright State University School of Medicine, Dayton, OH 45435, USA

Received April 28, 2004; Revised June 3, 2004; Accepted June 15, 2004

In addition to increased DNA-strand exchange, a cytogenetic feature of cells lacking the RecQ-like BLM helicase is a tendency for telomeres to associate. We also report additional cellular and biochemical evidence for the role of BLM in telomere maintenance. BLM co-localizes and complexes with the telomere repeat protein TRF2 in cells that employ the recombination-mediated mechanism of telomere lengthening known as ALT (alternative lengthening of telomeres). BLM co-localizes with TRF2 in foci actively synthesizing DNA during late S and G2/M; co-localization increases in late S and G2/M when ALT is thought to occur. Additionally, TRF1 and TRF2 interact directly with BLM and regulate BLM unwinding activity in vitro. Whereas TRF2 stimulates BLM unwinding of telomeric and non-telomeric substrates, TRF1 inhibits BLM unwinding of telomeric substrates only. Finally, TRF2 stimulates BLM unwinding with equimolar concentrations of TRF1, but not when TRF1 is added in molar excess. These data suggest a function for BLM in recombination-mediated telomere lengthening and support a model for the coordinated regulation of BLM activity at telomeres by TRF1 and TRF2.

* To whom correspondence should be addressed at: Department of Molecular Genetics, Biochemistry and Microbiology, Howard Hughes Medical Institute, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA. Tel: +1 5135580088; Email: joanna.groden{at}uc.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
N. Selak, C. Z. Bachrati, I. Shevelev, T. Dietschy, B. van Loon, A. Jacob, U. Hubscher, J. D. Hoheisel, I. D. Hickson, and I. Stagljar
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase {delta}
Nucleic Acids Res., September 1, 2008; 36(16): 5166 - 5179.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. M. Brosh Jr and V. A. Bohr
Human premature aging, DNA repair and RecQ helicases
Nucleic Acids Res., December 3, 2007; 35(22): 7527 - 7544.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
V. A. Rao, C. Conti, J. Guirouilh-Barbat, A. Nakamura, Z.-H. Miao, S. L. Davies, B. Sacca, I. D. Hickson, A. Bensimon, and Y. Pommier
Endogenous {gamma}-H2AX-ATM-Chk2 Checkpoint Activation in Bloom's Syndrome Helicase Deficient Cells Is Related to DNA Replication Arrested Forks
Mol. Cancer Res., July 1, 2007; 5(7): 713 - 724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Fouche, A. J. Cesare, S. Willcox, S. Ozgur, S. A. Compton, and J. D. Griffith
The Basic Domain of TRF2 Directs Binding to DNA Junctions Irrespective of the Presence of TTAGGG Repeats
J. Biol. Chem., December 8, 2006; 281(49): 37486 - 37495.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Fouche, S. Ozgur, D. Roy, and J. D. Griffith
Replication fork regression in repetitive DNAs
Nucleic Acids Res., November 6, 2006; 34(20): 6044 - 6050.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Sekiguchi, T. Hayano, M. Yanagida, N. Takahashi, and T. Nishimoto
NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47
Nucleic Acids Res., September 11, 2006; 34(16): 4593 - 4608.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. P. Killoran and J. L. Keck
Sit down, relax and unwind: structural insights into RecQ helicase mechanisms
Nucleic Acids Res., September 10, 2006; 34(15): 4098 - 4105.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. S. O'Connor, A. Safari, H. Xin, D. Liu, and Z. Songyang
A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly
PNAS, August 8, 2006; 103(32): 11874 - 11879.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Azam, J. Y. Lee, V. Abraham, R. Chanoux, K. A. Schoenly, and F. B. Johnson
Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence
Nucleic Acids Res., January 20, 2006; 34(2): 506 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Muftuoglu, H. K. Wong, S. Z. Imam, D. M. Wilson III, V. A. Bohr, and P. L. Opresko
Telomere Repeat Binding Factor 2 Interacts with Base Excision Repair Proteins and Stimulates DNA Synthesis by DNA Polymerase {beta}
Cancer Res., January 1, 2006; 66(1): 113 - 124.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. L. Opresko, P. A. Mason, E. R. Podell, M. Lei, I. D. Hickson, T. R. Cech, and V. A. Bohr
POT1 Stimulates RecQ Helicases WRN and BLM to Unwind Telomeric DNA Substrates
J. Biol. Chem., September 16, 2005; 280(37): 32069 - 32080.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Lillard-Wetherell, K. A. Combs, and J. Groden
BLM Helicase Complements Disrupted Type II Telomere Lengthening in Telomerase-Negative sgs1 Yeast
Cancer Res., July 1, 2005; 65(13): 5520 - 5522.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.