Skip Navigation


Human Molecular Genetics Advance Access originally published online on February 12, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
13/7/741    most recent
ddh081v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Li, T.
Right arrow Articles by Hoffman, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, T.
Right arrow Articles by Hoffman, A. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2004, Vol. 13, No. 7 741-750
DOI: 10.1093/hmg/ddh081

Activating and silencing histone modifications form independent allelic switch regions in the imprinted Gnas gene

Tao Li{dagger}, Thanh H. Vu{dagger}, Gary A. Ulaner, Youwen Yang, Ji-Fan Hu and Andrew R. Hoffman*

Medical Service, Veteran Affairs Palo Alto Health Care System, and Department of Medicine, Stanford University, Palo Alto, CA 94304, USA

Received December 5, 2003; Accepted January 30, 2004

Activation and suppression of gene transcription is tightly controlled by epigenetic modifications. The imprinted Gnas1 gene region contains closely juxtaposed maternally expressed (Nesp) and paternally expressed (Nespas, Gnasxl, Exon 1A) transcripts, providing a unique opportunity to study how epigenetic modifications change in nucleosomes from active to silenced promoters. Using 30 polymorphic sites across the Gnas1 gene region in (C57BL/6JxMus spretus) F1 mice and chromatin immunoprecipitation (ChIP) assays we identified two allelic switch regions (ASRs) that mark boundaries of epigenetic information. We show that activating signals (histone acetylation and methylation of H3 Lys4) and silencing signals (histone methylation of H3 Lys9 and DNA methylation) segregate independently across the ASRs and suggest that these ASRs allow the transcriptional elongation to proceed through the silenced domain of nearby imprinted promoters. We discuss these findings in light of recent progress in the conceptualization of nucleosome remodeling during transcriptional elongation and in the development of histone code.

* To whom correspondence should be addressed. Tel: +1 6504935000 ext. 63930; Fax: +1 6508568024; Email: arhoffman{at}stanford.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
Y. Yamasaki-Ishizaki, T. Kayashima, C. K. Mapendano, H. Soejima, T. Ohta, H. Masuzaki, A. Kinoshita, T. Urano, K.-i. Yoshiura, N. Matsumoto, et al.
Role of DNA Methylation and Histone H3 Lysine 27 Methylation in Tissue-Specific Imprinting of Mouse Grb10
Mol. Cell. Biol., January 15, 2007; 27(2): 732 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
T. Li, T. H. Vu, G. A. Ulaner, E. Littman, J.-Q. Ling, H.-L. Chen, J.-F. Hu, B. Behr, L. Giudice, and A. R. Hoffman
IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch
Mol. Hum. Reprod., September 1, 2005; 11(9): 631 - 640.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. X. Jin, Y.-W. Leu, S. Liyanarachchi, H. Sun, M. Fan, K. P. Nephew, T. H.-M. Huang, and R. V. Davuluri
Identifying estrogen receptor {alpha} target genes using integrated computational genomics and chromatin immunoprecipitation microarray
Nucleic Acids Res., December 17, 2004; 32(22): 6627 - 6635.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
T. H. Vu, T. Li, and A. R. Hoffman
Promoter-restricted histone code, not the differentially methylated DNA regions or antisense transcripts, marks the imprinting status of IGF2R in human and mouse
Hum. Mol. Genet., October 1, 2004; 13(19): 2233 - 2245.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.