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Human Molecular Genetics Advance Access originally published online on November 10, 2004
Human Molecular Genetics 2005 14(1):85-93; doi:10.1093/hmg/ddi008
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Human Molecular Genetics, Vol. 14, No. 1 © Oxford University Press 2005; all rights reserved

Variable and hierarchical size distribution of L1-retroelement-enriched CENP-A clusters within a functional human neocentromere

Anderly C. Chueh, Lee H. Wong, Nicholas Wong and K.H. Andy Choo*

Chromosome Research Laboratory, Murdoch Childrens Research Institute, Melbourne University Department of Paediatrics, Royal Children's Hospital, Parkville 3052, Australia

* To whom correspondence should be addressed. Tel: +61 383416306; Fax: +61 393481391; Email: andy.choo{at}mcri.edu.au

Received August 25, 2004; Revised October 13, 2004; Accepted October 25, 2004

Human neocentromeres are fully functional centromeres that arise epigenetically from non-centromeric precursor sequences that are devoid of {alpha}-satellite DNA. Using chromatin immunoprecipitation (ChIP) and BAC-array analysis, we have previously described a 330 kb binding domain for CENP-A (a histone H3 variant that confers centromere-specific nucleosomal property) at the 10q25 neocentromere found on a chromosome 10-derived marker chromosome mardel(10). For the further detailed analysis of the CENP-A-associated chromatin, we have generated a high-resolution genomic array consisting of PCR fragments with an average size of 8 kb, providing an ~20-fold increment in analytical resolution. ChIP and PCR-array analysis reveals seven distinct CENP-A-binding clusters within the 330 kb domain, demonstrating the interspersion of CENP-A-associated nucleosomal blocks within the neocentromeric chromatin. Independent ChIP–PCR analysis verified this distribution profile and indicated that histone H3-containing nucleosomes directly intervene the CENP-A-binding clusters. The CENP-A-binding clusters are uneven in size, with the central cluster (>50 kb) being significantly larger than the flanking ones (10–30 kb), and the flanking clusters arranged in an interesting hierarchical and symmetrical configuration of alternating larger and smaller sizes around the central cluster. In silico sequence analysis indicates an ~2.5-fold increase in the prevalence of L1 retroelements within the CENP-A-binding clusters when compared with the non-CENP-A-binding regions. These results provide insight into the possible role of retroelements in determining the positioning of CENP-A binding at human neocentromeres, and that a hierarchical and symmetrical arrangement of CENP-A-binding clusters of varying sizes may be an important structural requirement for mammalian kinetochore assembly and/or to provide stability to withstand polar microtubule forces.


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