Skip Navigation


Human Molecular Genetics Advance Access originally published online on September 23, 2005
Human Molecular Genetics 2005 14(21):3237-3248; doi:10.1093/hmg/ddi354
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
14/21/3237    most recent
ddi354v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kulpa, D. A.
Right arrow Articles by Moran, J. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kulpa, D. A.
Right arrow Articles by Moran, J. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Ribonucleoprotein particle formation is necessary but not sufficient for LINE-1 retrotransposition

Deanna A. Kulpa1 and John V. Moran1,2,*

1Department of Human Genetics and 2Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA

* To whom correspondence should be addressed at: 1241 E. Catherine, 4909 Buhl, University of Michigan, Ann Arbor, MI 48109, USA. Tel: +1 7346150456; Fax: +1 7347633784; Email: moranj{at}umich.edu

Received June 16, 2005; Revised August 17, 2005; Accepted September 15, 2005

Long interspersed elements (LINE-1s or L1s) are abundant non-LTR retrotransposons that mobilize through an RNA intermediate by target site primed reverse transcription. The L1-encoded proteins (ORF1p and ORF2p) preferentially associate with their encoding transcript to form a ribonucleoprotein particle (RNP), which is a proposed retrotransposition intermediate. Here, we have used epitope tagging to discriminate the proteins encoded by engineered L1s from those encoded by endogenously expressed L1s. We demonstrate that an L1 containing an epitope tag at the carboxyl terminus of ORF1p remains retrotransposition-competent and that tagged ORF1p and its encoding RNA localize to cytoplasmic RNPs. We also identified two classes of ORF1p mutants, one that severely decreased RNP formation and blocked retrotransposition, and another that allows RNP formation but reduces retrotransposition by 100-fold. Thus, these data indicate that RNP formation is important but not sufficient for L1 retrotransposition and suggest that ORF1p also may function at downstream steps in the L1 retrotransposition pathway.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
V. P. Belancio, D. J. Hedges, and P. Deininger
Mammalian non-LTR retrotransposons: For better or worse, in sickness and in health
Genome Res., March 1, 2008; 18(3): 343 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Chung, O. Siol, T. Dingermann, and T. Winckler
Protein Interactions Involved in tRNA Gene-Specific Integration of Dictyostelium discoideum Non-Long Terminal Repeat Retrotransposon TRE5-A
Mol. Cell. Biol., December 15, 2007; 27(24): 8492 - 8501.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Januszyk, P. W.-l. Li, V. Villareal, D. Branciforte, H. Wu, Y. Xie, J. Feigon, J. A. Loo, S. L. Martin, and R. T. Clubb
Identification and Solution Structure of a Highly Conserved C-terminal Domain within ORF1p Required for Retrotransposition of Long Interspersed Nuclear Element-1
J. Biol. Chem., August 24, 2007; 282(34): 24893 - 24904.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. E. Dmitriev, D. E. Andreev, I. M. Terenin, I. A. Olovnikov, V. S. Prassolov, W. C. Merrick, and I. N. Shatsky
Efficient Translation Initiation Directed by the 900-Nucleotide-Long and GC-Rich 5' Untranslated Region of the Human Retrotransposon LINE-1 mRNA Is Strictly Cap Dependent Rather than Internal Ribosome Entry Site Mediated
Mol. Cell. Biol., July 1, 2007; 27(13): 4685 - 4697.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. L. Garcia-Perez, M. C.N. Marchetto, A. R. Muotri, N. G. Coufal, F. H. Gage, K. S. O'Shea, and J. V. Moran
LINE-1 retrotransposition in human embryonic stem cells
Hum. Mol. Genet., July 1, 2007; 16(13): 1569 - 1577.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. L. Garcia-Perez, A. J. Doucet, A. Bucheton, J. V. Moran, and N. Gilbert
Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase
Genome Res., May 1, 2007; 17(5): 602 - 611.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Muckenfuss, M. Hamdorf, U. Held, M. Perkovic, J. Lower, K. Cichutek, E. Flory, G. G. Schumann, and C. Munk
APOBEC3 Proteins Inhibit Human LINE-1 Retrotransposition
J. Biol. Chem., August 4, 2006; 281(31): 22161 - 22172.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Matsumoto, M. Hamada, M. Osanai, and H. Fujiwara
Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1.
Mol. Cell. Biol., July 1, 2006; 26(13): 5168 - 5179.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Kubo, M. d. C. Seleme, H. S. Soifer, J. L. G. Perez, J. V. Moran, H. H. Kazazian Jr., and N. Kasahara
L1 retrotransposition in nondividing and primary human somatic cells
PNAS, May 23, 2006; 103(21): 8036 - 8041.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. d. C. Seleme, M. R. Vetter, R. Cordaux, L. Bastone, M. A. Batzer, and H. H. Kazazian Jr.
Extensive individual variation in L1 retrotransposition capability contributes to human genetic diversity
PNAS, April 25, 2006; 103(17): 6611 - 6616.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. P. Belancio, D. J. Hedges, and P. Deininger
LINE-1 RNA splicing and influences on mammalian gene expression
Nucleic Acids Res., March 22, 2006; 34(5): 1512 - 1521.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. W.-L. Li, J. Li, S. L. Timmerman, L. A. Krushel, and S. L. Martin
The dicistronic RNA from the mouse LINE-1 retrotransposon contains an internal ribosome entry site upstream of each ORF: implications for retrotransposition
Nucleic Acids Res., February 6, 2006; 34(3): 853 - 864.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. S. Alisch, J. L. Garcia-Perez, A. R. Muotri, F. H. Gage, and J. V. Moran
Unconventional translation of mammalian LINE-1 retrotransposons
Genes & Dev., January 15, 2006; 20(2): 210 - 224.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.