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Human Molecular Genetics Advance Access originally published online on September 28, 2007
Human Molecular Genetics 2007 16(23):2972-2986; doi:10.1093/hmg/ddm278
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© The Author 2007. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Adipose tissue mass is modulated by SLUG (SNAI2)

Pedro Antonio Pérez-Mancera1, Camino Bermejo-Rodríguez1, Inés González-Herrero1, Michel Herranz1, Teresa Flores2, Rafael Jiménez3 and Isidro Sánchez-García1,*

1 Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer (IBMCC), CSIC, 2 Servicio de Anatomía Patológica and 3 Departamento de Fisiología y Farmacología, Universidad de Salamanca, Campus M. Unamuno, 37007 Salamanca, Spain

* To whom correspondence should be addressed. Tel: +34 923238403; Fax: +34 923294813; Email: isg{at}usal.es

Received July 13, 2007; Revised September 5, 2007; Accepted September 19, 2007

The zinc-finger transcription factor SLUG (SNAI2) triggers epithelial–mesenchymal transitions (EMTs) and plays an important role in the developmental processes. Here, we show that SLUG is expressed in white adipose tissue (WAT) in humans and its expression is tightly controlled during adipocyte differentiation. Slug-deficient mice exhibit a marked deficiency in WAT size, and Slug-overexpressing mice (Combi-Slug) exhibit an increase in the WAT size. Consistent with in vivo data, Slug-deficient mouse embryonic fibroblasts (MEFs) showed a dramatically reduced capacity for adipogenesis in vitro and there was extensive lipid accumulation in Combi-Slug MEFs. The analysis of adipogenic gene expression both in vivo and in vitro showed that peroxisome proliferator-activated factor {gamma}2 (PPAR{gamma}2) expression was altered. Complementation studies rescued this phenotype, indicating that WAT alterations induced by Slug are reversible. Our results further show a differential histone deacetylase recruitment to the PPAR{gamma}2 promoter in a tissue- and Slug-dependent manner. Our results connect, for the first time, adipogenesis with the requirement of a critical level of an EMT regulator in mammals. This work may lead to the development of targeted drugs for the treatment of patients with obesity and/or lipodystrophy.


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