Human Molecular Genetics Advance Access originally published online on March 11, 2008
Human Molecular Genetics 2008 17(12):1867-1875; doi:10.1093/hmg/ddn082
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Identification of DIO2 as a new susceptibility locus for symptomatic osteoarthritis
,*1 Department of Molecular Epidemiology 2 Department of Rheumatology 3 Department of Medical Statistics and Bioinformatics 4 Department of Radiology 5 Department of Clinical Epidemiology and Haematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands 6 Department of Internal Medicine 7 Department of Epidemiology and Biostatistics, Erasmus University Medical School, 3015 GE Rotterdam, The Netherlands 8 Pfizer Research Technology Center, Cambridge, MA 02139, USA 9 Nuffield Department of Orthopaedic Surgery, Institute of Musculoskeletal Sciences, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, UK 10 Laboratory for Bone and Joint Diseases, SRC, RIKEN, University of Tokyo, Minato-ku, Tokyo, Japan
* To whom correspondence should be addressed at: Department of Molecular Epidemiology, Leiden University Medical Centre, Postzone S-05-P, PO Box 9600, 2300 RC Leiden, The Netherlands. Tel: +31 715269734; Fax: +31 715268280; E-mail: i.meulenbelt{at}lumc.nl
Received February 7, 2008; Accepted March 9, 2008
Osteoarthritis [MIM 165720 [OMIM] ] is a common late-onset articular joint disease for which no pharmaceutical intervention is available to attenuate the cartilage degeneration. To identify a new osteoarthritis susceptibility locus, a genome-wide linkage scan and combined linkage association analysis were applied to 179 affected siblings and four trios with generalized osteoarthritis (The GARP study). We tested, for confirmation by association, 1478 subjects who required joint replacement and 734 controls in a UK population. Additional replication was tested in 1582 population-based females from the Rotterdam study that contained 94 cases with defined hip osteoarthritis and in 267 Japanese females with symptomatic hip osteoarthritis and 465 controls. Suggested evidence for linkage in the GARP study was observed on chromosome 14q32.11 (log of odds = 3.03, P = 1.9x10–4). Genotyping tagging single-nucleotide polymorphisms covering three important candidate genes revealed a common coding variant (rs225014; Thr92Ala) in the iodothyronine-deiodinase enzyme type 2 (D2) gene (DIO2 [MIM 601413 [OMIM] ]) which significantly explained the linkage signal (P = 0.006). Confirmation and replication by association in the additional osteoarthritis studies indicated a common DIO2 haplotype, exclusively containing the minor allele of rs225014 and common allele of rs12885300, with a combined recessive odds ratio of 1.79, 95% confidence interval (CI) 1.37–2.34 with P = 2.02x10–5 in female cases with advanced/symptomatic hip osteoarthritis. The gene product of this DIO2 converts intracellular pro-hormone-3,3',5,5'-tetraiodothyronine (T4) into the active thyroid hormone 3,3',5-triiodothyronine (T3) thereby regulating intracellular levels of active T3 in target tissues such as the growth plate. Our results indicate a new susceptibility gene (DIO2) conferring risk to osteoarthritis.
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.
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