© 1993 Oxford University Press
RESEARCH-ARTICLE |
Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization
Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of lowa College of Medicine lowa City, IA 52242 1Department of Genetics and Pediatrics, Stanford University School of Medicine Stanford, CA, USA 2Howard Hughes Medical Institute, Stanford University School of Medicine Stanford, CA, USA
*To whom correspondence should be addressed at: Howard Hughes Medical Institute, University of Iowa College of Medicine, 400 EMRB, Iowa City, IA 52242, USA
Received June 1, 1993; Revised July 19, 1993; Accepted July 19, 1993
Dystroglycan is a novel laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Here we report the cDNA and genomic structure of human dystroglycan. The human dystroglycan is encoded by a single gene (DAG1) mapped to chromosome 3 band p21. The coding sequence is organized into two exons, separated by a large intron. The predicted amino acid sequence of human and rabbit dystroglycan are 93% IdentIcal with predicted glycosylatlon sites being conserved. Human dystroglycan is expressed in a variety of fetal and adult tissues. Our data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore, we hypothesize that variable glycosylatlon of the conserved protein core might modulate laminin binding. The relationship of dystroglycan to human diseases is discussed.
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|
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|
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|
|
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|
|
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|
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|
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|
|
|
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|
|
|
|
|
|
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|