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© 1993 Oxford University Press

RESEARCH-ARTICLE

construction of a transcription map of a 300 kb region around the human G6PD locus by direst cDNA selection

Zdenek Sediacek, Bernhard Korn, David S. Konecki, Renate Siebenhaar, Johannes F. Coy, Petra Kioschis and Annemarie Poustka*

Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506 69120 Herdelberg, Germany

*To whom correspondence should be addressed

Received July 8, 1993; Revised September 7, 1993; Accepted September 7, 1993

A transcription map covering a 300 kb region around the G6PD gene in the human Xq28 region was constructed by the direct cDNA selection method and the analysis of the resulting region-specific enriched cDNA subllbrary. Seven new genes and two loci of endogenous retrovirus HERV-K were identified. The distribution of the genes across the region is strongly non-uniform and follows the non-uniform distribution of CpG islands in the area. While one of the novel genes was found to be highly homologous to bovine smg p25A GDP-dissociation inhibitor, the remaining genes did not detect any homology to known genes. The analysis of region-specific cDNA subilbraries represents a simple, rapid and efficient tool for the generation of a regional transcription map.


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 Genome ResHome page
N S Heiss, U C Rogner, P Kioschis, B Korn, and A Poustka
Transcription mapping in a 700-kb region around the DXS52 locus in Xq28: isolation of six novel transcripts and a novel ATPase isoform (hPMCA5).
Genome Res., June 1, 1996; 6(6): 478 - 491.
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