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© 1993 Oxford University Press

RESEARCH-ARTICLE

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

Michael R. Spelcher*, Stanislas du Manoir, Evelln SchrÖck, Heidi Holtgreve-Grez, Brlgltte Schoell, Christoph Lengauer{dagger}, Thomas Cremer and Thomas Ried

Institut für Humangenetik, Universtät Hedelberg Im Neuenhermer Feld 328, D-69120 Heidelberg, Germany

*To whom correspondence should be addressed

Received June 25, 1993; Revised August 29, 1993; Accepted August 29, 1993

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications In archived solid tumor samples.

{dagger}Present address: Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, A-1030 Wien, Austria


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