© 1993 Oxford University Press
RESEARCH-ARTICLE |
The human SRY transcript
1Department of Paediatrics and Endocnnology, Royal Children's Hospital Victona 3052, Australia 2Department of Biochemistry and Genetics, St Mary's Hospital London 3Laboratory of Eucaryotic Molecular Genetics, MRC National Institute for Medical Research The Ridgeway, Mill Hill, London NW7 1AA, UK
*To whom correspondence should be addressed
Received July 22, 1993; Revised October 12, 1993; Accepted October 12, 1993
SRY encodes the Y-linked testis-determining factor in humans. A predominant 900 bp transcript originates from a single exon and encompasses the putative SRY coding sequence. We show that in human adult testis SRY transcription involves multiple start sites. In addition to a previously defined major initiation site, transcripts originating at least 410 bp upstream of this site were detected. Using a cDNA specific RT - PCR assay, embryonic and adult human tissues were screened for SRY expression. In humans, SRY transcription is not restricted to the presumptive and the mature gonadal tissues In the embryo and the adult respectively but can be detected In a range of other locations. Two human cell lines, NTERA-2 cl.D1 (NT2/D1) and Hep G2, have been Identified which express SRY at similar levels to adult testis. The NT2/D1 SRY transcripts appear to have the same structure as those in adult testis. HMBA-Induced differentiation of NT2/D1 cells results in a diminution of SRY mRNA, while transcription of SRY In retinoic acid differentiated NT2/D1 Is unaffected.
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