Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Costes, B.
Right arrow Articles by Goossens, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Costes, B.
Right arrow Articles by Goossens, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1993 Oxford University Press

OTHER

Psoralen-modified oligonucleotide primers improve detection of mutations by denaturing gradient gel electrophoresis and provide an alternative to GC-clamping

B. Costes, E. Girodon, N. Ghanem, M. Chassignol1, N.T. Thuong1, D. Dupret2 and M. Goossens*

INSERM U91, Ho{ring}pital Henri Mondor F-94010 Créteil cedex 1Centre de Biophysique Moléculaire F-45071 Orléans cedex 2 2Laboratoire de Biologie Moléculaire Appliquée Appligene, F-67402 Illkirch cedex, France

* To whom correspondence should be addressed

Received December 14, 1992; Revised February 10, 1993; Accepted February 10, 1993

Denaturing gradient gel electrophoresis (DGGE), a mutation-scanning procedure separating DNA fragments differing by as little as a single base change, is widely used in studies of genomic nucleotide sequence variability. The efficiency of the technique is greatly enhanced by attaching, through polymerase chain reaction (PCR) incorporation, a long GC-tail to the test DNA sequence which, as a result, becomes analysable throughout. As synthesis of GC-rich specific PCR primers is costly and time-consuming, we attempted to clamp the DNA fragment using a psoralen derivative (ChemiClamp) that promotes photo-induced cross-linking at one end. We found that this procedure provides an attractive alternative to GC-clamp in DGGE (and temperature gradient gel electrophoresis) and should prove useful in both research and diagnostic laboratories.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin Med ResHome page
R. J. Wurzburger, R. Gupta, A. P. Parnassa, S. Jain, J. A. Wexler, J. L. Chu, K. B. Elkon, and R. D. Blank
Use of GC Clamps in DHPLC Mutation Scanning
Clin. Med. Res., April 1, 2003; 1(2): 111 - 118.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
J.-P. Mira, A. Cariou, F. Grall, C. Delclaux, M.-R. Losser, F. Heshmati, C. Cheval, M. Monchi, J.-L. Teboul, F. Riche, et al.
Association of TNF2, a TNF-{alpha} Promoter Polymorphism, With Septic Shock Susceptibility and Mortality: A Multicenter Study
JAMA, August 11, 1999; 282(6): 561 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. Moatti, P. Seknadji, C. Galand, O. Poirier, F. Fumeron, S. Desprez, M. Garbarz, D. Dhermy, D. Arveiler, A. Evans, et al.
Polymorphisms of the Tissue Factor Pathway Inhibitor (TFPI) Gene in Patients With Acute Coronary Syndromes and in Healthy Subjects : Impact of the V264M Substitution on Plasma Levels of TFPI
Arterioscler Thromb Vasc Biol, April 1, 1999; 19(4): 862 - 869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Laderman, J. R. Penny, F. Mazzucchelli, N. Bresolin, G. Scarlato, and G. Attardi
Aging-dependent Functional Alterations of Mitochondrial DNA (mtDNA) from Human Fibroblasts Transferred into mtDNA-less Cells
J. Biol. Chem., July 5, 1996; 271(27): 15891 - 15897.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.