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© 1994 Oxford University Press

OTHER

A high resolution physical map of 2.5 Mbp of the Down syndrome region on chromosome 21

Nila Patil, Andrew Peterson1, Alana Rothman1, Pieter J.de Jong2,+, Richard M. Myers and David R. Cox*,

Department of Genetics, Stanford University School of Medicine Stanford, CA 94305-5120 1Department of Physiology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0444 2Biomedical Sciences Division, Lawrence Livermore National Laboratory Livermore, CA 94550, USA

*To whom correspondence should be addressed

Received June 10, 1994; Revised August 15, 1994; Accepted August 15, 1994

The region surrounding D21S55 in band 21q22 of human chromosome 21 has been implicated in the etiology of Down syndrome (DS). In this paper, we report the construction of a high resolution map of a 2.5 Mb region around the marker D21S55. Characterization of YAC clones by accurate size determination, end isolation and marker assignment was used to build a refined YAC-based map. The YAC clones were then used to isolate 284 cosmid clones, covering 2.3 Mb, from a chromosome 21-specific cosmid library. The cosmid clones were ordered into overlapping groups and a restriction map of each group was determined. The groups of cosmids were then ordered and oriented with respect to the YAC-based map. This high resolution map provides the framework for further analysis of the region by transcribed sequence mapping and sequence determination.


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