© 1994 Oxford University Press
OTHER |
An integrated YAC-overlap and ‘cosmid-pocket’ map of the human chromosome 21
eti
*Genome Analyse Laboratory, Imperial Cancer Research Fund, P. O Box 123 Lincoln's Inn Fields London WC2A 3PX, UK 1Instrtut Alfred Fessard, CNRS, Avenue de la Terrasse, 91198 Grf-sur-Yvette Cedex 2URA CNRS 1335, Hopital Necker, 149 Rue de Sevres, 75743 Paris Cedex 15 France 3Department of Biochemistry, University of Antwerp, Unrversfteteplein 1, B-2610 Antwerp Belgium
*To whom correspondence should be addressed
Received February 8, 1994; Accepted March 23, 1994
We describe here the construction of an ordered clone map of human chromosome 21, based on the identification of ordered sets of YAC clones covering >90% of the chromosome, and their use to identify groups of cosmid clones (cosmid pockets) localised to subregions defined by the YAC clone map. This Is to our knowledge the highest resolution map of one human chromosome to date, localising 530 YAC clones covering both arms of the chromosome, spanning >36 Mbp, and localising more than 6300 cosmlds to 145 intervals on both arms of the chromosome. The YAC contigs have been formed by hybridising a 6. 1 equivalents chromosome 21 enriched YAC collection displayed on arrayed nylon membranes to a series of 115 DNA markers and Alu-PCR products from YACs. Forty eight mega-YACs from the previously published CEPH-Genethon map of sequence tagged sites (STS) have also been Included in the contig building experiments. A YAC tiling path was then size-measured and confirmed by gel-fingerprinting. A minimal tiling path of 70 YACs were then used as probes against the 7. 5 genome equivalents flow sorted chromosome 21 cosmid library in order to identify the lists of cosmids mapping to alternating shared - non-shared intervals between overlapping YACs (‘cosmid pockets’). For approximately 1/5 of the minimal tiling path of YACs, locations and non-chlmaerism have been confirmed by fluorescence in situ hybridisation (FISH), and approximately 1/5 of all cosmid pocket assignments have independent, confirmatory marker hybridisations in the ICRF cosmid reference library system. We also demonstrate that ‘pockets’ contain overlapping sets of cosmids (cosmid contigs). In addition to being an important logical intermediate step between the YAC maps published so far and a future map of completely ordered cosmids, this map provides immediately available low-complexity cosmid material for high resolution FISH mapping of chromosomal aberrations on Interphase nuclei, and for rapid positional isolation of transcripts In the highly resolved regions of genetic interest.
+These authors have contributed equally to this work
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-U. G. Weier DNA Fiber Mapping Techniques for the Assembly of High-resolution Physical Maps J. Histochem. Cytochem., August 1, 2001; 49(8): 939 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. NADAL, C. G. VIGO, M. I. MELARAGNO, J. A D ANDRADE, L. G. ALONSO, D. BRUNONI, M. PRITCHARD, and X. ESTIVILL Clinical and cytogenetic characterisation of a patient with Down syndrome resulting from a 21q22.1{right-arrow}qter duplication J. Med. Genet., January 1, 2001; 38(1): 73 - 76. [Full Text]
|
|
|
|
 |
|
 E. T. Peterson, R. Sutherland, D. L. Robinson, L. Chasteen, M. Gersh, J. Overhauser, L. L. Deaven, R. K. Moyzis, and D. L. Grady An Integrated Physical Map for the Short Arm of Human Chromosome 5 Genome Res., December 1, 1999; 9(12): 1250 - 1267. [Abstract] [Full Text]
|
|
|
|
|
|
S.-Y. Wang, M. Cruts, J. Del-Favero, Y. Zhang, F. Tissir, M.-C. Potier, D. Patterson, D. Nizetic, A. Bosch, H. Chen, et al. A High-Resolution Physical Map of Human Chromosome 21p Using Yeast Artificial Chromosomes Genome Res., November 1, 1999; 9(11): 1059 - 1073. [Abstract] [Full Text]
|
|
|
|
|
|
T. Hildmann, X. Kong, J. O'Brien, L. Riesselman, H.-M. Christensen, E. Dagand, H. Lehrach, and M.-L. Yaspo A Contiguous 3-Mb Sequence-Ready Map in the S3-MX Region on 21q22.2 Based on High- Throughput Nonisotopic Library Screenings Genome Res., April 1, 1999; 9(4): 360 - 372. [Abstract] [Full Text]
|
|
|
|
|
|
J. Groet, J. H. Ives, A. P. South, P. R. Baptista, T. A. Jones, M.-L. Yaspo, H. Lehrach, M.-C. Potier, and C. Van Broeckhoven Bacterial Contig Map of the 21q11 Region Associated with Alzheimer's Disease and Abnormal Myelopoiesis in Down Syndrome Genome Res., April 1, 1998; 8(4): 385 - 398. [Abstract] [Full Text]
|
|
|
|
|
|
J. Aaltonen, N. Horelli-Kuitunen, J.-B. Fan, P. Bjorses, J. Perheentupa, R. Myers, A. Palotie, and L. Peltonen High-Resolution Physical and Transcriptional Mapping of the Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy Locus on Chromosome 21q22.3 by FISH Genome Res., August 1, 1997; 7(8): 820 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Chen, R Chrast, C Rossier, M A Morris, M D Lalioti, and S E Antonarakis Cloning of 559 potential exons of genes of human chromosome 21 by exon trapping. Genome Res., August 1, 1996; 6(8): 747 - 760. [Abstract] [PDF]
|
|
|
|
|
|
G Wohr, T Fink, and G Assum A palindromic structure in the pericentromeric region of various human chromosomes. Genome Res., April 1, 1996; 6(4): 267 - 279. [Abstract] [PDF]
|
|
|
|
|
|
J R Korenberg, X N Chen, S Mitchell, S Fannin, S Gerwehr, D Cohen, and I Chumakov A high-fidelity physical map of human chromosome 21q in yeast artificial chromosomes. Genome Res., December 1, 1995; 5(5): 427 - 443. [Abstract] [PDF]
|
|
|
|
|
|
M G Olavesen, A F Davies, S J Broxholme, J L Wixon, G Senger, D Nizetic, R D Campbell, and J Ragoussis An integrated map of human chromosome 6p23. Genome Res., November 1, 1995; 5(4): 342 - 358. [Abstract] [PDF]
|
|