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© 1994 Oxford University Press

OTHER

Analysis of the mouse and rat CFTR promoter regions

Erick Denamur and Farid F. Chehab*

Department of Laboratory Medicine, University of California San Francisco, CA 94143-0134, USA

*To whom correspondence should be addressed

Received February 22, 1994; Revised May 13, 1994; Accepted May 13, 1994

To gain insights into the regulation of the mouse and rat cystic fibrosis transmembrane conductance regulator (CFTR) genes, we cloned and sequenced their respective upstream promoter regions. DNA sequence analysis from either side of exon 1 revealed a complete divergence from the human DNA sequence except for three DNA motifs which consist of a 34 bp stretch and two intron specific elements. Highlights of both the rat and mouse promoter sequences include an extensive purine rich stretch, a Y-box motif and putative Sp1, AP1 sites. Transfection of mouse promoter deletional constructs into expressing and non-expressing CFTR murine cell lines revealed that the Pu.Py stretch and the Y-box act, respectively, as negative and positive elements of basal transcription that confer no apparent tissue specificity.


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