© 1995 Oxford University Press
Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers
Children's Hospital of Philadelphia, Division of Human Genetics and Molecular Biology, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104 1University of Pennsylvania School of Medicine, 415 Curie Boulevard Philadelphia, PA 19104–6146 2Fox Chase Cancer Center 7701 Burholme Avenue, Philadelphia, PA 19111–2412 3Center for Genome Research, Whitehead Institute for Biological Sciences/Massachusetts Institute of Technology 9 Cambridge Center, Cambridge, MA 02142, USA 4Department of Biological Sciences, University of Alberta Edmonton, Alberta T6G 2E9, Canada
*To whom correspondence should be addressed
Received November 2, 1994; Revised November 2, 1994; Accepted November 2, 1994
Detailed physical maps of the human genome are important resources for the Identification and isolation of disease genes and for studying the structure and function of the genome. We used data from STS content mapping of YACs and natural and induced chromosomal breakpoints to anchor contigs of overlapping yeast artificial chromosome (YAC) clones spanning extensive regions of human chromosome 22. The STSs were assigned to specific regions (bins) on the chromosome using cell lines from a somatic hybrid mapping panel defining a maximum of 25 intervals. YAC libraries were screened by PCR amplification of hierarchical pools of yeast DNA with 238 markers, and a total of 587 YAC clones were identified. These YACs were assembled into contigs based upon their shared STS content using a simulated annealing algorithm. Fifteen contigs, containing between 2 and 74 STSs were assembled, and ordered along the chromosome based upon the cytogenetic breakpoint, meiotic and PFG maps. Additional singleton YACs were assigned to unique chromosomal bins. These ordered YAC contigs will be useful for identifying disease genes and chromosomal breakpoints by positional cloning and will provide the foundation for higher resolution physical maps for large scale sequencing of the chromosome.
+Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Parkville 3050, Australia and
Genetic Disease Research NCHGR/NIH, 9000 Rockville Pike, 49/4A72, Bethesda, MD 20892, USA
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