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© 1995 Oxford University Press

OTHER

Expression of the myotonin protein kinase gene in preimplantation human embryos

Robert Daniels*, Thannasis Kinis1, Paul Serhal1 and Marilyn Monk

Molecular Embryology Unit, Institute of Child Health, University of London 30 Guilford Street, London WC1N 1EH UK 1Assisted Conception Unit, Department of Obstetrics and Gynaecology 25 Gratton Way, London WC1E 6DB, UK

*To whom correspondence should be addressed

Received August 9, 1994; Revised December 5, 1994; Accepted December 5, 1994

We have examined the expression of the myotonin protein kinase gene (MPK) in individual human preimplantation embryos. An allele-specific, reverse transcriptase polymerase chain reaction assay was developed to the required sensitivity using human buccal cells. Human cleavage stage embryos, derived by in vitro fertilisation, were assayed for MPK transcription directly, or after biopsy of a single blasto mere used to determine the genotype of the embryo. The maternal and paternal genotypes were determined from cumulus cells and sperm, respectively. MPK gene transcripts were detected in embryos at the 1-cell-4-cell stage of development. Detection of transcripts from the paternally-inherited allele confirmed onset of embryonic MPK gene expression at this very early preimplantation stage.


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