Human Molecular Genetics, Vol 5, 1801-1807, Copyright © 1996 by Oxford University Press
F Schnieders, T Dork, J Arnemann, T Vogel, M Werner and J Schmidtke
TSPY, the 'testis-specific protein, Y-encoded', is the product of a tandem
gene cluster on human proximal Yp. In order to gain insight into the
function of this locus, we have analysed (I) the diversity of RNAs
transcribed from the cluster, (II) the sequence homology of the deduced
TSPY to other proteins, and (iii) its protein properties both in tissue
extracts and in tissue sections, using a TSPY-specific antiserum. We have
identified a set of distinct TSPY transcripts with diverse exon
compositions. We show that TSPY has homology with other human and non-
human proteins, including SET and NAP, factors that are suggested to play a
role in DNA replication. Protein analysis revealed TSPY to occur mainly in
a modified, putatively phosphorylated form. By immunostaining it was
detected in distinct subsets of spermatogonia. TSPY was also strongly
immunostained in early testicular carcinoma in situ (CIS), while
seminomatous tumour cells stained less intensely. The spermatogonial cells
of two XY-TFM-females gave a strong immune response. The data presented
here point to a phosphorylation-dependent TSPY-function in early
spermatogenesis, immediately prior to the spermatogonia-to-spermatocyte
transition, and in early testicular tumorigenesis.
ARTICLES
Testis-specific protein, Y-encoded (TSPY) expression in testicular tissues
Institut fur Humangenetik, Medizinische Hochschule Hannover, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Giachini, F. Nuti, D. J. Turner, I. Laface, Y. Xue, F. Daguin, G. Forti, C. Tyler-Smith, and C. Krausz TSPY1 Copy Number Variation Influences Spermatogenesis and Shows Differences among Y Lineages J. Clin. Endocrinol. Metab., October 1, 2009; 94(10): 4016 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schubert, K. Kamino, D. Bohm, I. Adham, W. Engel, R. v. Wasielewski, D. Moharregh-Khiabani, G. Mauceri, B. Vaske, A. Meinhardt, et al. TSPY Expression Is Variably Altered in Transgenic Mice with Testicular Feminization Biol Reprod, July 1, 2008; 79(1): 125 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.C. Lardone, D.A. Parodi, R. Valdevenito, M. Ebensperger, A. Piottante, M. Madariaga, R. Smith, R. Pommer, N. Zambrano, and A. Castro Quantification of DDX3Y, RBMY1, DAZ and TSPY mRNAs in testes of patients with severe impairment of spermatogenesis Mol. Hum. Reprod., October 1, 2007; 13(10): 705 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Bhowmick, Y. Satta, and N. Takahata The origin and evolution of human ampliconic gene families and ampliconic structure Genome Res., April 1, 2007; 17(4): 441 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sun, S. Goodison, J. Li, L. Liu, and W. Farmerie Improved breast cancer prognosis through the combination of clinical and genetic markers Bioinformatics, January 1, 2007; 23(1): 30 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cools, S. L. S. Drop, K. P. Wolffenbuttel, J. W. Oosterhuis, and L. H. J. Looijenga Germ Cell Tumors in the Intersex Gonad: Old Paths, New Directions, Moving Frontiers Endocr. Rev., August 1, 2006; 27(5): 468 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cools, H. Stoop, A.-M. F. Kersemaekers, S. L. S. Drop, K. P. Wolffenbuttel, J.-P. Bourguignon, J. Slowikowska-Hilczer, K. Kula, S. M. H. Faradz, J. W. Oosterhuis, et al. Gonadoblastoma Arising in Undifferentiated Gonadal Tissue within Dysgenetic Gonads J. Clin. Endocrinol. Metab., June 1, 2006; 91(6): 2404 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Rajpert-De Meyts Developmental model for the pathogenesis of testicular carcinoma in situ: genetic and environmental aspects Hum. Reprod. Update, May 1, 2006; 12(3): 303 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. M. Gallagher, O. E. Bergin, M. Rafferty, Z. D. Kelly, I.-M. Nolan, E. J.P. Fox, A. C. Culhane, L. McArdle, M. F. Fraga, L. Hughes, et al. Multiple markers for melanoma progression regulated by DNA methylation: insights from transcriptomic studies Carcinogenesis, November 1, 2005; 26(11): 1856 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cools, K. van Aerde, A.-M. Kersemaekers, M. Boter, S. L. S. Drop, K. P. Wolffenbuttel, E. W. Steyerberg, J. W. Oosterhuis, and L. H. J. Looijenga Morphological and Immunohistochemical Differences between Gonadal Maturation Delay and Early Germ Cell Neoplasia in Patients with Undervirilization Syndromes J. Clin. Endocrinol. Metab., September 1, 2005; 90(9): 5295 - 5303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Puffenberger, D. Hu-Lince, J. M. Parod, D. W. Craig, S. E. Dobrin, A. R. Conway, E. A. Donarum, K. A. Strauss, T. Dunckley, J. F. Cardenas, et al. Mapping of sudden infant death with dysgenesis of the testes syndrome (SIDDT) by a SNP genome scan and identification of TSPYL loss of function PNAS, August 10, 2004; 101(32): 11689 - 11694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Umehara and M. Horikoshi Transcription Initiation Factor IID-interactive Histone Chaperone CIA-II Implicated in Mammalian Spermatogenesis J. Biol. Chem., September 12, 2003; 278(37): 35660 - 35667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schubert, B. Skawran, F. Dechend, K. Nayernia, A. Meinhardt, I. Nanda, M. Schmid, W. Engel, and J. Schmidtke Generation and Characterization of a Transgenic Mouse with a Functional Human TSPY Biol Reprod, September 1, 2003; 69(3): 968 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Quintana-Murci, M E Weale, M G Thomas, E Erdei, N Bradman, J H Shanks, C Krausz, and K McElreavey Y chromosome haplotypes and testicular cancer in the English population J. Med. Genet., March 1, 2003; 40(3): e20 - 20. [Full Text] [PDF]
|
|
|
|
|
|
E. Makrinou, M. Fox, M. Lovett, K. Haworth, J. M. Cameron, K. Taylor, and Y. H. Edwards TTY2: A Multicopy Y-Linked Gene Family Genome Res., June 1, 2001; 11(6): 935 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tascou, K. Nayernia, A. Samani, J. Schmidtke, T. Vogel, W. Engel, and P. Burfeind Immortalization of Murine Male Germ Cells at a Discrete Stage of Differentiation by a Novel Directed Promoter-Based Selection Strategy Biol Reprod, November 1, 2000; 63(5): 1555 - 1561. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Elliott, K Oghene, G Makarov, O Makarova, T. Hargreave, A. Chandley, I. Eperon, and H. Cooke Dynamic changes in the subnuclear organisation of pre-mRNA splicing proteins and RBM during human germ cell development J. Cell Sci., January 5, 1998; 111(9): 1255 - 1265. [Abstract] [PDF]
|
|