Human Molecular Genetics, Vol 5, 1851-1858, Copyright © 1996 by Oxford University Press
YN Li, S Gulati, PJ Baker, LC Brody, R Banerjee and WD Kruger
Elevated levels of plasma homocysteine is a risk factor in both birth
defects and vascular disease. Methionine synthase (MS) is a cobalamin
dependent enzyme which catalyzes methylation of homocysteine to methionine.
Impaired MS activity is expected to lead to increased levels of plasma
homocysteine. In addition, defects in this gene may underlie the
methionine-dependence observed in a number of human tumor cell lines. We
describe here the isolation and characterization of the human MS cDNA. It
contains an open reading frame of 3798 nucleotides encoding a protein of
1265 amino acids with a predicted molecular mass of 140 kDa. The amino acid
sequence of the human MS is 55% identical with that of the Escherichia coli
enzyme (METH) and 64% identical with the predicted Caenorhabditis elegans
enzyme. Seven peptide sequences derived from purified porcine MS have
substantial similarity to the human protein. Northern analysis indicates
that the MS RNA is present in a wide variety of tissues. We have mapped the
human gene to chromosomal location 1q43, a region found monosomic in
individuals with deletion 1q syndrome. The isolation of the MS cDNA will
now allow the direct determination of whether mutations in this gene
contribute to folate-related neural tube defects, cardiovascular diseases,
and birth defects.
ARTICLES
Cloning, mapping and RNA analysis of the human methionine synthase gene
Division of Population Science, Fox Chase Cancer Center, Philadelphia, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. M. Frizzell, M. J. Gamble, J. G. Berrocal, T. Zhang, R. Krishnakumar, Y. Cen, A. A. Sauve, and W. L. Kraus Global Analysis of Transcriptional Regulation by Poly(ADP-ribose) Polymerase-1 and Poly(ADP-ribose) Glycohydrolase in MCF-7 Human Breast Cancer Cells J. Biol. Chem., December 4, 2009; 284(49): 33926 - 33938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garg, V. Vitvitsky, H. E. Gendelman, and R. Banerjee Monocyte Differentiation, Activation, and Mycobacterial Killing Are Linked to Transsulfuration-dependent Redox Metabolism J. Biol. Chem., December 15, 2006; 281(50): 38712 - 38720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tamura and M. F. Picciano Folate and human reproduction Am. J. Clinical Nutrition, May 1, 2006; 83(5): 993 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhang, Q. Shi, Z. Liu, E. M. Sturgis, M. R. Spitz, and Q. Wei Polymorphisms of Methionine Synthase and Methionine Synthase Reductase and Risk of Squamous Cell Carcinoma of the Head and Neck: a Case-Control Analysis Cancer Epidemiol. Biomarkers Prev., May 1, 2005; 14(5): 1188 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Olteanu and R. Banerjee Redundancy in the Pathway for Redox Regulation of Mammalian Methionine Synthase: REDUCTIVE ACTIVATION BY THE DUAL FLAVOPROTEIN, NOVEL REDUCTASE 1 J. Biol. Chem., October 3, 2003; 278(40): 38310 - 38314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Oltean and R. Banerjee Nutritional Modulation of Gene Expression and Homocysteine Utilization by Vitamin B12 J. Biol. Chem., May 30, 2003; 278(23): 20778 - 20784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Carmel, R. Green, D. S. Rosenblatt, and D. Watkins Update on Cobalamin, Folate, and Homocysteine Hematology, January 1, 2003; 2003(1): 62 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Dobson, T. Wai, D. Leclerc, H. Kadir, M. Narang, J. P. Lerner-Ellis, T. J. Hudson, D. S. Rosenblatt, and R. A. Gravel Identification of the gene responsible for the cblB complementation group of vitamin B12-dependent methylmalonic aciduria Hum. Mol. Genet., December 15, 2002; 11(26): 3361 - 3369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Dobson, T. Wai, D. Leclerc, A. Wilson, X. Wu, C. Dore, T. Hudson, D. S. Rosenblatt, and R. A. Gravel Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements PNAS, November 26, 2002; 99(24): 15554 - 15559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M.J. van der Put, H. W.M. van Straaten, F. J.M. Trijbels, and H. J. Blom Folate, Homocysteine and Neural Tube Defects: An Overview Experimental Biology and Medicine, April 1, 2001; 226(4): 243 - 270. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. A. Swanson, M.-L. Liu, P. J. Baker, L. Garrett, M. Stitzel, J. Wu, M. Harris, R. Banerjee, B. Shane, and L. C. Brody Targeted Disruption of the Methionine Synthase Gene in Mice Mol. Cell. Biol., February 15, 2001; 21(4): 1058 - 1065. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. L JOHANNING, T TAMURA, K. E JOHNSTON, and K. D WENSTROM Comorbidity of 5,10-methylenetetrahydrofolate reductase and methionine synthase gene polymorphisms and risk for neural tube defects J. Med. Genet., December 1, 2000; 37(12): 949 - 951. [Full Text]
|
|
|
|
 |
|
 B. Tang, Y. N. Li, and W. D. Kruger Defects in Methylthioadenosine Phosphorylase Are Associated with but not Responsible for Methionine-dependent Tumor Cell Growth Cancer Res., October 1, 2000; 60(19): 5543 - 5547. [Abstract] [Full Text]
|
|
|
|
|
|
K. Yamada, S. Yamada, T. Tobimatsu, and T. Toraya Heterologous High Level Expression, Purification, and Enzymological Properties of Recombinant Rat Cobalamin-dependent Methionine Synthase J. Biol. Chem., December 10, 1999; 274(50): 35571 - 35576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Jacobsen Homocysteine and vitamins in cardiovascular disease Clin. Chem., August 1, 1998; 44(8): 1833 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Leclerc, A. Wilson, R. Dumas, C. Gafuik, D. Song, D. Watkins, H. H. Q. Heng, J. M. Rommens, S. W. Scherer, D. S. Rosenblatt, et al. Cloning and mapping of a cDNA for methionine synthase reductase, a flavoprotein defective in patients with homocystinuria PNAS, March 17, 1998; 95(6): 3059 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gulati, Z. Chen, L. C. Brody, D. S. Rosenblatt, and R. Banerjee Defects in Auxiliary Redox Proteins Lead to Functional Methionine Synthase Deficiency J. Biol. Chem., August 1, 1997; 272(31): 19171 - 19175. [Abstract] [Full Text] [PDF]
|
|