Human Molecular Genetics, Vol 5, 809-813, Copyright © 1996 by Oxford University Press
F Tamanini, N Meijer, C Verheij, PJ Willems, H Galjaard, BA Oostra and AT Hoogeveen
The FMR1 transcript is alternatively spliced and generates different splice
variants coding for FMR1 proteins (FMRP) with a predicted molecular mass of
70-80 kDa. FMRP is widely expressed and localized in the cytoplasm. To
study a possible interaction with other cellular components, FMRP was
isolated and characterized under non-denaturing conditions. Under
physiological salt conditions FMRP appears to have a molecular mass of >
600 kDa, indicating a binding to other cellular components. This
interaction is disrupted in the presence of high salt concentrations. The
dissociation conditions to free FMRP from the complex are similar to the
dissociation of FMRP from RNA as shown before. The binding of FMRP from the
complex is also disrupted by RNAse treatment. That the association of FMRP
to a high molecular weight complex possibly occurs via RNA, is further
supported by the observation that the binding of FMRP, containing an
lle304Asn substitution, to the high molecular weight complex is reduced. An
equal reduced binding of mutated FMRP to RNA in vitro was observed before
under the same conditions. The reduced binding of FMRP with the lle304Asn
substitution further indicates that the interaction to the complex indeed
occurs via FMRP and not via other RNA binding proteins. In a reconstitution
experiment where the low molecular mass FMRP (70-80 kDa) is mixed with a
reticulocyte lysate (enriched in ribosomes) it was shown that FMRP can
associate to ribosomes and that this binding most likely occurs via RNA.
ARTICLES
FMRP is associated to the ribosomes via RNA
MGC-Department of Clinical Genetics, Erasmus University, Rotterdam, The Netherlands.
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