Human Molecular Genetics, Vol 6, 2031-2041, Copyright © 1997 by Oxford University Press
D Heckel, N Brass, U Fischer, N Blin, I Steudel, O Tureci, O Fackler, KD Zang and E Meese
There is increasing evidence that tumor expressed genes induce immune
responses in cancer patients. To identify meningioma expressed antigens, we
established a meningioma expression library which was screened with
autologous serum. Out of 20 positive cDNA clones eight share high sequence
homologies as determined by sequence analysis. These eight clones can be
grouped into three classes which differ in length and which are
characterized by specific sequence variations. The longest open reading
frame was found to be 2412 bp encoding an immunoreactive antigen termed
meningioma expressed antigen 6 (MEA6). Using five sequence specific primer
pairs, somatic hybrid panel mapping revealed locations of the three classes
on several human chromosomes including chromosomes 2, 3, 6, 7, 9, 13 and
14. The mapping results were confirmed by fluorescence in situ
hybridization. RT-PCR showed consistent expression of all classes in
several meningiomas and additional tissues using the same set of primer
pairs as for chromosomal mapping. The expression data were confirmed by
northern blot analysis. For the predicted amino acid sequence BLASTX
revealed a homology to a human C219-reactive peptide which was previously
isolated by an antibody directed against p-glycoprotein. Sequence
properties of the MEA protein include an acidic activation domain, a
proline-rich region and two coiled-coil domains indicating protein binding
and activation functions.
ARTICLES
cDNA cloning and chromosomal mapping of a predicted coiled-coil proline- rich protein immunogenic in meningioma patients
Institut fur Humangenetik, Theoretische Medizin, Universitat des Saarlandes, Homburg/Saar, Germany.
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