Human Molecular Genetics, Vol 6, 2223-2231, Copyright © 1997 by Oxford University Press
K Yamada, M Matsuki, Y Morishima, E Ueda, K Tabata, H Yasuno, M Suzuki and K Yamanishi
Transglutaminase 1 (TGase 1) is a tissue-specific enzyme which is expressed
in the keratinized stratified squamous epithelia and which catalyzes
straightepsilon-(gamma-glutamyl) lysine cross-links of proteins to form the
cell envelope at the periphery of cornified cells. A transient expression
assay using a luciferase reporter gene linked to the 2.5 kb 5' upstream
region of the human TGase 1 gene (TGM1) showed phorbol ester-responsive
promoter activity in cultured normal human keratinocytes. To assess its
promoter activity in vivo, we generated transgenic mice expressing the
Escherichia coli beta-galactosidase gene (lacZ) directed by the 5' upstream
region. beta-Galactosidase histochemistry revealed that the TGM1-lacZ
transgene was expressed in terminally differentiating keratinocytes in
upper layers of stratified squamous epithelia in embryonic, neonatal and
adult transgenic mice. The expression pattern was similar to that of
endogenous TGase 1 mRNA detected by in situ hybridization. Furthermore,
topical application of a phorbol ester to adult tail skin enhanced
expression of the transgene as well as TGase 1 mRNA in the epidermis. Thus,
the 2.5 kb 5' upstream sequence of TGM1 includes elements regulating
tissue- and terminal differentiation-specific gene expression in stratified
squamous epithelia.
ARTICLES
Activation of the human transglutaminase 1 promoter in transgenic mice: terminal differentiation-specific expression of the TGM1-lacZ transgene in keratinized stratified squamous epithelia
Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. E. Iismaa, B. M. Mearns, L. Lorand, and R. M. Graham Transglutaminases and Disease: Lessons From Genetically Engineered Mouse Models and Inherited Disorders Physiol Rev, July 1, 2009; 89(3): 991 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Z. Chi, J.-O. Yang, K.-Y. Lee, K. Ito, C. Sakakura, Q.-L. Li, H.-R. Kim, E.-J. Cha, Y.-H. Lee, A. Kaneda, et al. RUNX3 Suppresses Gastric Epithelial Cell Growth by Inducing p21WAF1/Cip1 Expression in Cooperation with Transforming Growth Factor {beta}-Activated SMAD Mol. Cell. Biol., September 15, 2005; 25(18): 8097 - 8107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, J. Hicks, P. Khanbolooki, S.-J. Kim, C. Yan, Y. Wang, and D. Boyd Transgenic Mice Demonstrate Novel Promoter Regions for Tissue-Specific Expression of the Urokinase Receptor Gene Am. J. Pathol., August 1, 2003; 163(2): 453 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakamura, K. Nishida, A. Dota, M. Matsuki, K. Yamanishi, and S. Kinoshita Elevated Expression of Transglutaminase 1 and Keratinization-Related Proteins in Conjunctiva in Severe Ocular Surface Disease Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 549 - 556. [Abstract] [Full Text]
|
|
|
|
|
|
R. Inada, M. Matsuki, K. Yamada, Y. Morishima, S.-C. Shen, N. Kuramoto, H. Yasuno, K. Takahashi, Y. Miyachi, and K. Yamanishi Facilitated Wound Healing by Activation of the Transglutaminase 1 Gene Am. J. Pathol., December 1, 2000; 157(6): 1875 - 1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hiiragi, H. Sasaki, A. Nagafuchi, H. Sabe, S. C. Shen, M. Matsuki, K. Yamanishi, and S. Tsukita Transglutaminase Type 1 and Its Cross-linking Activity Are Concentrated at Adherens Junctions in Simple Epithelial Cells J. Biol. Chem., November 26, 1999; 274(48): 34148 - 34154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nishida, K. Yamanishi, K. Yamada, A. Dota, S. Kawasaki, A. J. Quantock, and S. Kinoshita Epithelial Hyperproliferation and Transglutaminase 1 Gene Expression in Stevens-Johnson Syndrome Conjunctiva Am. J. Pathol., February 1, 1999; 154(2): 331 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Crish, T. M. Zaim, and R. L. Eckert The Distal Regulatory Region of the Human Involucrin Promoter Is Required for Expression in Epidermis J. Biol. Chem., November 13, 1998; 273(46): 30460 - 30465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Matsuki, F. Yamashita, A. Ishida-Yamamoto, K. Yamada, C. Kinoshita, S. Fushiki, E. Ueda, Y. Morishima, K. Tabata, H. Yasuno, et al. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase) PNAS, February 3, 1998; 95(3): 1044 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maatta, C. Ruhrberg, and F. M. Watt Structure and Regulation of the Envoplakin Gene J. Biol. Chem., June 23, 2000; 275(26): 19857 - 19865. [Abstract] [Full Text] [PDF]
|
|