Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (61)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Fukagawa, T.
Right arrow Articles by Brown, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukagawa, T.
Right arrow Articles by Brown, W. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, Vol 6, 2301-2308, Copyright © 1997 by Oxford University Press

ARTICLES

Efficient conditional mutation of the vertebrate CENP-C gene

T Fukagawa and WR Brown
Cancer Research Campaign Chromosome Molecular Biology Group, Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, UK. tfukagaw@bioch.ox.ac.uk

We have used gene targeting in the DT40 cell line to create a cell line which expresses a fusion between CENP-C and a mouse steroid receptor and which behaves as a conditional loss of function mutant of CENP-C. Under restrictive conditions these cells arrest at the metaphase/anaphase junction and after a delay of approximately 2.5 h die by apoptosis. These results indicate that CENP-C is either necessary for anaphase chromosome movement or for mediating a signal which triggers centromere function during anaphase. Our approach is simple and applicable to a wide range of proteins with general cell autonomous functions in vertebrates.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
K. J. Milks, B. Moree, and A. F. Straight
Dissection of CENP-C-directed Centromere and Kinetochore Assembly
Mol. Biol. Cell, October 1, 2009; 20(19): 4246 - 4255.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M.-S. Kwon, T. Hori, M. Okada, and T. Fukagawa
CENP-C Is Involved in Chromosome Segregation, Mitotic Checkpoint Function, and Kinetochore Assembly
Mol. Biol. Cell, June 1, 2007; 18(6): 2155 - 2168.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Churikov, C. Wei, and C. M. Price
Vertebrate POT1 Restricts G-Overhang Length and Prevents Activation of a Telomeric DNA Damage Checkpoint but Is Dispensable for Overhang Protection.
Mol. Cell. Biol., September 1, 2006; 26(18): 6971 - 6982.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Minoshima, T. Hori, M. Okada, H. Kimura, T. Haraguchi, Y. Hiraoka, Y.-C. Bao, T. Kawashima, T. Kitamura, and T. Fukagawa
The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage
Mol. Cell. Biol., December 1, 2005; 25(23): 10315 - 10328.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Heeger, O. Leismann, R. Schittenhelm, O. Schraidt, S. Heidmann, and C. F. Lehner
Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
Genes & Dev., September 1, 2005; 19(17): 2041 - 2053.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Regnier, P. Vagnarelli, T. Fukagawa, T. Zerjal, E. Burns, D. Trouche, W. Earnshaw, and W. Brown
CENP-A Is Required for Accurate Chromosome Segregation and Sustained Kinetochore Association of BubR1
Mol. Cell. Biol., May 15, 2005; 25(10): 3967 - 3981.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Mikami, T. Hori, H. Kimura, and T. Fukagawa
The Functional Region of CENP-H Interacts with the Nuf2 Complex That Localizes to Centromere during Mitosis
Mol. Cell. Biol., March 1, 2005; 25(5): 1958 - 1970.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Maiato, J. DeLuca, E. D. Salmon, and W. C. Earnshaw
The dynamic kinetochore-microtubule interface
J. Cell Sci., November 1, 2004; 117(23): 5461 - 5477.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Sanyal, M. Baum, and J. Carbon
Centromeric DNA sequences in the pathogenic yeast Candida albicans are all different and unique
PNAS, August 3, 2004; 101(31): 11374 - 11379.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Suzuki, M. Nakano, N. Nozaki, S.-i. Egashira, T. Okazaki, and H. Masumoto
CENP-B Interacts with CENP-C Domains Containing Mif2 Regions Responsible for Centromere Localization
J. Biol. Chem., February 13, 2004; 279(7): 5934 - 5946.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Tawaramoto, S.-Y. Park, Y. Tanaka, O. Nureki, H. Kurumizaka, and S. Yokoyama
Crystal Structure of the Human Centromere Protein B (CENP-B) Dimerization Domain at 1.65-A Resolution
J. Biol. Chem., December 19, 2003; 278(51): 51454 - 51461.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Hori, T. Haraguchi, Y. Hiraoka, H. Kimura, and T. Fukagawa
Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
J. Cell Sci., August 15, 2003; 116(16): 3347 - 3362.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Saxena, R. Saffery, L. H. Wong, P. Kalitsis, and K. H. A. Choo
Centromere Proteins Cenpa, Cenpb, and Bub3 Interact with Poly(ADP-ribose) Polymerase-1 Protein and Are Poly(ADP-ribosyl)ated
J. Biol. Chem., July 19, 2002; 277(30): 26921 - 26926.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Ando, H. Yang, N. Nozaki, T. Okazaki, and K. Yoda
CENP-A, -B, and -C Chromatin Complex That Contains the I-Type {alpha}-Satellite Array Constitutes the Prekinetochore in HeLa Cells
Mol. Cell. Biol., April 1, 2002; 22(7): 2229 - 2241.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Politi, G. Perini, S. Trazzi, A. Pliss, I. Raska, W. C. Earnshaw, and G. D. Valle
CENP-C binds the alpha-satellite DNA in vivo at specific centromere domains
J. Cell Sci., January 6, 2002; 115(11): 2317 - 2327.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Fukagawa, V. Regnier, and T. Ikemura
Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells
Nucleic Acids Res., September 15, 2001; 29(18): 3796 - 3803.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. L. Moore and M. B. Roth
Hcp-4, a Cenp-C-Like Protein inCaenorhabditis elegans, Is Required for Resolution of Sister Centromeres
J. Cell Biol., June 11, 2001; 153(6): 1199 - 1208.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. A. Van Hooser, I. I. Ouspenski, H. C. Gregson, D. A. Starr, T. J. Yen, M. L. Goldberg, K. Yokomori, W. C. Earnshaw, K. F. Sullivan, and B. R. Brinkley
Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A
J. Cell Sci., January 10, 2001; 114(19): 3529 - 3542.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
I. Abdrakhmanov, D. Lodygin, P. Geroth, H. Arakawa, A. Law, J. Plachy, B. Korn, and J.-M. Buerstedde
A Large Database of Chicken Bursal ESTs as a Resource for the Analysis of Vertebrate Gene Function
Genome Res., December 1, 2000; 10(12): 2062 - 2069.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
A. Pluta, W. Earnshaw, and I. Goldberg
Interphase-specific association of intrinsic centromere protein CENP-C with HDaxx, a death domain-binding protein implicated in Fas-mediated cell death
J. Cell Sci., June 8, 2000; 111(14): 2029 - 2041.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. V. Howman, K. J. Fowler, A. J. Newson, S. Redward, A. C. MacDonald, P. Kalitsis, and K. H. A. Choo
Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice
PNAS, February 1, 2000; 97(3): 1148 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
E. Earle, A. Saxena, A. MacDonald, D. F. Hudson, L. G. Shaffer, R. Saffery, M. R. Cancilla, S. M. Cutts, E. Howman, and K. H. A. Choo
Poly(ADP-ribose) polymerase at active centromeres and neocentromeres at metaphase
Hum. Mol. Genet., January 22, 2000; 9(2): 187 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. W.I. Lo, G. C.-C. Liao, M. Rocchi, and K.H. A. Choo
Extreme Reduction of Chromosome-Specific alpha -Satellite Array Is Unusually Common in Human Chromosome 21
Genome Res., October 1, 1999; 9(10): 895 - 908.
[Abstract] [Full Text]

Home page
 Plant CellHome page
R. K. Dawe, L. M. Reed, H.-G. Yu, M. G. Muszynski, and E. N. Hiatt
A Maize Homolog of Mammalian CENPC Is a Constitutive Component of the Inner Kinetochore
PLANT CELL, July 1, 1999; 11(7): 1227 - 1238.
[Abstract] [Full Text]

Home page
 JCBHome page
D. F. Hudson, K. J. Fowler, E. Earle, R. Saffery, P. Kalitsis, H. Trowell, J. Hill, N. G. Wreford, D. M. de Kretser, M. R. Cancilla, et al.
Centromere Protein B Null Mice are Mitotically and Meiotically Normal but Have Lower Body and Testis Weights
J. Cell Biol., April 20, 1998; 141(2): 309 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Yoda, S. Ando, S. Morishita, K. Houmura, K. Hashimoto, K. Takeyasu, and T. Okazaki
Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro
PNAS, June 20, 2000; 97(13): 7266 - 7271.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.