Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (48)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shen, M. H.
Right arrow Articles by Brown, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, M. H.
Right arrow Articles by Brown, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, Vol 6, 1375-1382, Copyright © 1997 by Oxford University Press

ARTICLES

Human mini-chromosomes in mouse embryonal stem cells

MH Shen, J Yang, ML Loupart, A Smith and W Brown
Cancer Research Campaign Chromosome Molecular Biology Group, Biochemistry Department, Oxford University, UK.

We have introduced human mini-chromosomes of 4 Mb and approximately 15 Mb in size into mouse embryonal stem cells. Although these human mini- chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection. However, one of the mini-chromosomes re- arranged, acquired mouse centromeric sequences and was then stably maintained for at least 60 population doublings in culture. This mini- chromosome, which is 4 Mb in size, is a candidate for a mouse germ line chromosome vector.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Suzuki, K. Nishii, T. Okazaki, and M. Ikeno
Human Artificial Chromosomes Constructed Using the Bottom-up Strategy Are Stably Maintained in Mitosis and Efficiently Transmissible to Progeny Mice
J. Biol. Chem., September 8, 2006; 281(36): 26615 - 26623.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Malla, F. Dafhnis-Calas, J. F. Y. Brookfield, M. C. M. Smith, and W. R. A. Brown
Rearranging the centromere of the human Y chromosome with {varphi}C31 integrase
Nucleic Acids Res., October 24, 2005; 33(19): 6101 - 6113.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. H. Wong, R. Saffery, M. A. Anderson, E. Earle, J. M. Quach, A. J. Stafford, K. J. Fowler, and K. H. A. Choo
Analysis of Mitotic and Expression Properties of Human Neocentromere-based Transchromosomes in Mice
J. Biol. Chem., February 4, 2005; 280(5): 3954 - 3962.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
T. Voet, J. Vermeesch, A. Carens, J. Dürr, C. Labaere, H. Duhamel, G. David, and P. Marynen
Efficient Male and Female Germline Transmission of a Human Chromosomal Vector in Mice
Genome Res., January 1, 2001; 11(1): 124 - 136.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
J.W. Yang, C. Pendon, J. Yang, N. Haywood, A. Chand, and W.R.A. Brown
Human mini-chromosomes with minimal centromeres
Hum. Mol. Genet., July 22, 2000; 9(12): 1891 - 1902.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Tomizuka, T. Shinohara, H. Yoshida, H. Uejima, A. Ohguma, S. Tanaka, K. Sato, M. Oshimura, and I. Ishida
Double trans-chromosomic mice: Maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies
PNAS, January 18, 2000; 97(2): 722 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Burns, L Christopoulou, P Corish, and C Tyler-Smith
Quantitative measurement of mammalian chromosome mitotic loss rates using the green fluorescent protein
J. Cell Sci., January 8, 1999; 112(16): 2705 - 2714.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Saffery, L. H. Wong, D. V. Irvine, M. A. Bateman, B. Griffiths, S. M. Cutts, M. R. Cancilla, A. C. Cendron, A. J. Stafford, and K. H. A. Choo
From the Cover: Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation
PNAS, May 8, 2001; 98(10): 5705 - 5710.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.