Human Molecular Genetics, Vol 6, 1435-1443, Copyright © 1997 by Oxford University Press
I Danko, P Williams, H Herweijer, G Zhang, JS Latendresse, I Bock and JA Wolff
There is a time window at 2 weeks of age for achieving very high levels of
foreign gene expression from the intramuscular injection of naked plasmid
DNA in mice and rats. The highest expression, over 1 microg of luciferase
protein/muscle, was obtained in Balb/C mice using constructs containing the
CMV promoter, a chimeric intron and the luc+ luciferase gene. Approximately
50% of the myofibers were intensely blue following the intramuscular
injection of a beta-galactosidase expression vector in 2 week old Balb/C
mice. The effects of age, mouse strain and construct were multiplicative,
resulting in >1000-fold greater luciferase and approximately 20-fold
more beta-galactosidase-positive cells. These high levels of expression
were unstable and were not observed in larger animals (dog, rhesus monkey).
These results indicate that enormous levels of foreign gene expression can
be obtained in muscle with naked DNA in vivo and will enable the temporary
effects of gene function and expression in rodent muscle to be
expeditiously studied.
ARTICLES
High expression of naked plasmid DNA in muscles of young rodents
University of Wisconsin-Madison, Department of Pediatrics, Waisman Center, 53705, USA.
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