Human Molecular Genetics, Vol 6, 1549-1558, Copyright © 1997 by Oxford University Press
NR dos Santos, DR de Bruijn, M Balemans, B Janssen, F Gartner, JM Lopes, B de Leeuw and A Geurts van Kessel
Synovial sarcoma is characterized by a prevalent chromosomal translocation,
t(X;18)(p11;q11). As a result of this translocation the SYT gene on
chromosome 18 fuses to either the SSX1 or the SSX2 gene on the X
chromosome. In this study, we generated polyclonal antibodies against the
SYT and SSX2 proteins. These antibodies specifically detected both these
proteins and the SYT-SSX fusion proteins in transfected COS-1 cell
extracts. Indirect immunofluorescence analysis of COS-1 cells expressing
tagged or untagged SYT, SSX2, SYT-SSX1 or SYT- SSX2 indicated that all
these proteins are localized in the nucleus, excluding the nucleoli. The
SSX2 protein exhibited a diffuse staining pattern whereas both the SYT and
SYT-SSX proteins appeared in several nuclear dots. Similar nuclear dots
were also detected in primary synovial sarcoma cells growing in a
short-term in vitro culture. Double immunofluorescence in conjunction with
confocal laser-scanning microscopy revealed that the SYT and SYT-SSX
nuclear dots do not co- localize with known nuclear structures as e.g.
coiled bodies, SC35 interchromatin granules or PML bodies. The similar
nuclear localization patterns of SYT and SYT-SSX suggest that the SYT-SSX
fusion proteins are directed to SYT-associated nuclear domains where an
abnormal function may be exerted.
ARTICLES
Nuclear localization of SYT, SSX and the synovial sarcoma-associated SYT-SSX fusion proteins
Department of Human Genetics, University Hospital Nijmegen, The Netherlands. n.dossantos@antrg.azn.nl
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