Human Molecular Genetics, Vol 7, 1967-1978, Copyright © 1998 by Oxford University Press
MS Ko, TA Threat, X Wang, JH Horton, Y Cui, X Wang, E Pryor, J Paris, J Wells- Smith, JR Kitchen, LB Rowe, J Eppig, T Satoh, L Brant, H Fujiwara, S Yotsumoto and H Nakashima
Mammalian embryos can only survive if they attach to the uterus
(implantation) and establish proper maternal-fetal interactions. To
understand this complex implantation pathway, we have initiated genomic
analysis with a systematic study of the cohort of genes expressed in
extraembryonic cells that are derived from the conceptus and play a major
role in this process. A total of 2103 cDNAs from the extraembryonic portion
of 7.5-day post-conception mouse embryos yielded 3186 expressed sequence
tags, approximately 40% of which were novel to the sequence databases.
Furthermore, when 155 of the cDNA clones with no homology to previously
detected genes were genetically mapped, apparent clustering of these
expressed genes was detected in subregions of chromosomes 2, 7, 9 and 17,
with 6.5% of the observed genes localized in the t-complex region of
chromosome 17, which represents only approximately 1.5% of the mouse
genome. In contrast, X-linked genes were under-represented.
Semi-quantitative RT-PCR analyses of the mapped genes demonstrated that one
third of the genes were expressed solely in extraembryonic tissue and an
additional one third of the genes were expressed predominantly in the
extraembryonic tissues. The over-representation of extraembryonic-expressed
genes in dosage- sensitive autosomal imprinted regions and
under-representation on the dosage-compensated X chromosome may reflect a
need for tight quantitative control of expression during development.
ARTICLES
Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome
Center for Molecular Medicine and Genetics and Department of Internal Medicine, Wayne State University School of Medicine, 5047 Gullen Mall, Detroit, MI 48202, USA. msko@cmb.biosci.wayne.edu
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