Human Molecular Genetics, Vol 7, 671-677, Copyright © 1998 by Oxford University Press
NE Faulkner, B Vig, CJ Echeverri, L Wordeman and RB Vallee
Multicentric chromosomes are often found in tumor cells and certain cell
lines. How they are generated is not fully understood, though their
stability suggests that they are non-functional during chromosome
segregation. Growing evidence has implicated microtubule motor proteins in
attachment of chromosomes to the mitotic spindle and in chromosome
movement. To better understand the molecular basis for the inactivity of
centromeres associated with secondary constrictions, we have tested these
structures by immunofluorescence microscopy for the presence of motor
complexes and associated proteins. We find strong immunoreactivity at the
active, but not inactive, centromeres of prometaphase multicentric
chromosomes using antibodies to the cytoplasmic dynein intermediate chains,
three components of the dynactin complex (dynamitin, Arp1 and p150 Glued ),
the kinesin-related proteins CENP-E and MCAK and the proposed structural
and checkpoint proteins HZW10, CENP-F and Mad2p. These results offer new
insight into the assembly and composition of both primary and secondary
constrictions and provide a molecular basis for the apparent inactivity of
the latter during chromosome segregation.
ARTICLES
Localization of motor-related proteins and associated complexes to active, but not inactive, centromeres
Cell Biology Group, Worcester Foundation for Biomedical Research, Shrewsbury, MA 01545, USA.
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