Human Molecular Genetics, Vol 7, 685-691, Copyright © 1998 by Oxford University Press
SR Twigg, HD Burns, M Oldridge, JK Heath and AO Wilkie
The two classes of sequences for recognition and splicing of pre-mRNA in
eukaryotes, GT-AG and AT-AC, are characterized by the nearly invariant
dinucleotides present at the extreme 5' (donor) and 3' (acceptor) ends of
the intron. Amongst GT-AG introns, which comprise the vast majority, the
more extended consensus sequence at the 5' splice site isACAG/GTAGAGT
(where / indicates the exon-intron boundary). This sequence is
complementary to part of the U1 snRNA and is important in intron
recognition. We have determined the genomic structure of the mouse
fibroblast growth factor receptor 2 gene (Fgfr2) and identified a divergent
5' splice site (ACA/GAAAGT), conserved in FGFR1 , - 2 and - 3 from humans,
mice and Xenopus that is used for alternative splicing of a hexanucleotide
sequence, encoding Val-Thr, at the end of exon 10. This is the only example
known of the use of /GA in vertebrate splicing. Similarities to a splice
site in the Antennapedia gene of Drosophila suggest that this variant motif
is involved in alternative splicing of short sequences at the 5' splice
site. Inclusion or exclusion of the Val-Thr dipeptide may play an important
role in controlling FGFR signalling through the Ras/MAPK pathway.
ARTICLES
Conserved use of a non-canonical 5' splice site (/GA) in alternative splicing by fibroblast growth factor receptors 1, 2 and 3
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
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