Human Molecular Genetics, Vol 7, 919-932, Copyright © 1998 by Oxford University Press
MQ Zhang
To facilitate gene finding and for the investigation of human molecular
genetics on a genome scale, we present a comprehensive survey on various
statistical features of human exons. We first show that human exons with
flanking genomic DNA sequences can be classified into 12 mutually exclusive
categories. This classification could serve as a standard for future
studies so that direct comparisons of results can be made. A database for
eight categories (related to human genes in which coding regions are split
by introns) was built from GenBank release 87.0 and analyzed by a number of
methods to characterize statistical features of these sequences that may
serve as controls or regulatory signals for gene expression. The
statistical information compiled includes profiles of signals for
transcription, splicing and translation, various compositional statistics
and size distributions. Further analyses reveal novel correlations and
constraints among different splicing features across an internal exon that
are consistent with the Exon Definition model. This information is
fundamental for a quantitative view of human gene organization, and should
be invaluable for individual scientists to design human molecular genetics
experiments.
ARTICLES
Statistical features of human exons and their flanking regions
Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, NY 11724, USA. mzhang@cshl.org
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