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Human Molecular Genetics, 2000, Vol. 9, No. 12 1721-1727
© 2000 Oxford University Press

The TSC1 gene product, hamartin, negatively regulates cell proliferation

Angelina Miloloza, Margit Rosner, Mark Nellist1, Dicky Halley1, Gerhard Bernaschek and Markus Hengstschläger+

Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Währinger Gürtel 18-20, A-1090 Vienna, Austria and 1MGC Department of Clinical Genetics, Erasmus University, 3015GE Rotterdam, The Netherlands

Tuberous sclerosis is an autosomal dominant hereditary disease caused by mutations in either the TSC1 or the TSC2 tumor suppressor gene. The TSC1 gene on chromosome 9q34 encodes a 130 kDa protein named hamartin, and the TSC2 gene on chromosome 16p13.3 codes for tuberin, a 200 kDa protein. Here we show that expression of hamartin, assayed by immunoblot analyses, is high in G0-arrested cells and hamartin is expressed throughout the entire ongoing cell cycle. An interaction of hamartin and tuberin can be detected in every phase of the cell cycle. Ectopic expression of high levels of hamartin attenuates cellular proliferation. We provide evidence that this effect could depend on a coiled-coil region earlier proposed to be involved in binding of hamartin to tuberin. Further investigations revealed that hamartin affects cell proliferation via deregulation of G1 phase. Our data have a clear impact on understanding the role of hamartin during development of this disease.

+ To whom correspondence should be addressed. Tel: +43 1 40400 7847; Fax: +43 1 40400 7848; Email: markus.hengstschlaeger@akh-wien.ac.at


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