Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Okabe, J.
Right arrow Articles by Nakanishi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okabe, J.
Right arrow Articles by Nakanishi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2000, Vol. 9, No. 18 2639-2650
© 2000 Oxford University Press

TRF1 is a critical trans-acting factor required for de novo telomere formation in human cells

Jun Okabe, Akiko Eguchi, Akinori Masago, Takao Hayakawa1 and Mahito Nakanishi+

Department of Neurovirology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan and 1Division of Biological Chemistry and Biologicals, National Institute of Health Science, Setagaya, Tokyo 158-8501, Japan

The duplex telomere repeat (TTAGGG)n is an essential cis-acting element of the mammalian telomere, and an exogenous telomere repeat can induce chromosome breakage and de novo telomere formation at the site of a break (telomere seeding). Telomere seeding requires the telomere repeat (TTAGGG)n more stringently than does an in vitro telomerase assay, suggesting that it reflects the activity of a critical trans-acting element of the functional telomere, in addition to telomerase. Furthermore, telomere seeding is induced at a frequency fluctuating widely among human cell lines, suggesting variation in the activity of this hypothetical factor among cells. In this study, we investigated the cellular factor(s) required for telomere formation using the frequency of telomere seeding as an index and identified TRF1, one of the telomere repeat binding proteins, as an essential trans-acting factor. The exogenous telomere repeat induces telomere formation at a frequency determined by the availability of TRF1, even in telomerase-negative cells. Our study shows clearly that TRF1 has a novel physiological significance distinct from its role as a regulator of telomere length in the endogenous chromosome. The possible role of TRF1 in cell aging and immortalization is discussed.

+ To whom correspondence should be addressed. Tel: +81 6 6879 8300; Fax: +81 6 6875 1170; Email: mahito@biken.osaka-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C. Cooley, K. M. Baird, V. Faure, T. Wenner, J. L. Stewart, S. Modino, P. Slijepcevic, C. J. Farr, and C. G. Morrison
Trf1 Is Not Required for Proliferation or Functional Telomere Maintenance in Chicken DT40 Cells
Mol. Biol. Cell, May 15, 2009; 20(10): 2563 - 2571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. H. Lee, K. Perrem, J. W. Harper, K. P. Lu, and X. Z. Zhou
The F-box Protein FBX4 Targets PIN2/TRF1 for Ubiquitin-mediated Degradation and Regulates Telomere Maintenance
J. Biol. Chem., January 13, 2006; 281(2): 759 - 768.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Basu, G. Stromberg, G. Compitello, H. F. Willard, and G. V. Bokkelen
Rapid creation of BAC-based human artificial chromosome vectors by transposition with synthetic alpha-satellite arrays
Nucleic Acids Res., January 26, 2005; 33(2): 587 - 596.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. O'Connor, A. Safari, D. Liu, J. Qin, and Z. Songyang
The Human Rap1 Protein Complex and Modulation of Telomere Length
J. Biol. Chem., July 2, 2004; 279(27): 28585 - 28591.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Okabe, A. Eguchi, R. Wadhwa, R. Rakwal, R. Tsukinoki, T. Hayakawa, and M. Nakanishi
Limited capacity of the nuclear matrix to bind telomere repeat binding factor TRF1 may restrict the proliferation of mortal human fibroblasts
Hum. Mol. Genet., February 1, 2004; 13(3): 285 - 293.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Ancelin, M. Brunori, S. Bauwens, C.-E. Koering, C. Brun, M. Ricoul, J.-P. Pommier, L. Sabatier, and E. Gilson
Targeting Assay To Study the cis Functions of Human Telomeric Proteins: Evidence for Inhibition of Telomerase by TRF1 and for Activation of Telomere Degradation by TRF2
Mol. Cell. Biol., May 15, 2002; 22(10): 3474 - 3487.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Eguchi, T. Akuta, H. Okuyama, T. Senda, H. Yokoi, H. Inokuchi, S. Fujita, T. Hayakawa, K. Takeda, M. Hasegawa, et al.
Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells
J. Biol. Chem., July 6, 2001; 276(28): 26204 - 26210.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.