Human Molecular Genetics, 2000, Vol. 9, No. 19 2821-2835
© 2000 Oxford University Press
Human mtDNA sublimons resemble rearranged mitochondrial genomes found in pathological states
1Institute of Medical Technology and 2Department of Forensic Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland, 3Department of Neurology, University of Oulu, Oulu, Finland, 4Department of Paediatrics, University of Oxford, Oxford, UK, 5MRC Dunn Human Nutrition Unit, Cambridge, UK and 6Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
Sublimons, originally identified in plant mitochondria, are defined as rearranged mtDNA molecules present at very low levels. We have analysed the primary structures of sublimons found in human cells and tissues and estimated their abundance. Each tissue of a given individual contains a wide range of different sublimons and the most abundant species differ between tissues in a substantially systematic manner. Sublimons are undetectable in 
0 cells, indicating that they are bona fide derivatives of mtDNA. They are most prominent in post-mitotic tissue subject to oxidative stress. Rearrangement break-points, often defined by short direct repeats, are scattered, but hotspot regions are clearly identifiable, notably near the end of the D-loop. The region between the replication origins is therefore frequently eliminated. One other hotspot region is located adjacent to a known site of protein binding, suggesting that recombination may be facilitated by protein–protein interactions. For a given primary rearrangement, both deleted and partially duplicated species can be detected. Although each sublimon is typically present at a low level, at most a few copies per cell, sublimon abundance in a given tissue can vary over three orders of magnitude between healthy individuals. Collectively, therefore, they can represent a non-negligible fraction of total mtDNA. Their structures are very similar to those of the rearranged molecules found in pathological states, such as adPEO and MNGIE; therefore, we propose that, as in plants, human mtDNA sublimons represent a pool of variant molecules that can become amplified under pathological conditions, thus contributing to cellular dysfunction.
+ To whom correspondence should be addressed at: Institute of Medical Technology, 33014 University of Tampere, Finland. Tel: +358 3 215 7731; Fax: +358 3 215 7731; Email: howy.jacobs@uta.fi
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