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Human Molecular Genetics, 2000, Vol. 9, No. 20 3091-3100
© 2000 Oxford University Press

A sarcoglycan–dystroglycan complex anchors Dp116 and utrophin in the peripheral nervous system

Michihiro Imamura1,+, Kenji Araishi1, Satoru Noguchi1,2 and Eijiro Ozawa1

1Department of Cell Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi-cho, Kodaira, Tokyo 187-8502, Japan and 2Inheritance and Variation Group, PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan

The dystrophin-associated membrane-integrated protein complex anchors dystrophin in the sarcolemma of striated muscles and is composed of two glycoprotein subcomplexes, the dystroglycan and the sarcoglycan (SG) complexes, and a small membrane protein termed sarcospan (SPN). The SG complex consists of four transmembrane glycoproteins, {alpha}-SG, ß-SG, {gamma}-SG and {delta}-SG. We found that ß-SG and {delta}-SG were co-expressed with {varepsilon}-SG, a {alpha}-SG homolog, in the peripheral nerve, but not with {alpha}-SG or {gamma}-SG. SPN, which tightly links to the SG complex in the muscle cell membrane, was absent in the peripheral nerve. These peripheral nerve SGs were colocalized at the outermost layer of the myelin sheath of nerve fibers together with the dystroglycan complex, utrophin, and a short dystrophin isoform (Dp116). Immunocytochemical analysis using SG-deficient animals showed that a defect in ß- or {delta}-SG led to a great reduction of all residual SGs, but not of the other proteins, i.e., dystroglycans, Dp116 and utrophin, in the peripheral nerve. This observation suggests that the {varepsilon}-, ß- and {delta}-SG molecules form a complex behaving as a single unit similar to the SG complex in muscle cells. An immunoprecipitation study indicated that the SG complex is associated with the dystroglycan complex and Dp116 or utrophin. These results demonstrated that Dp116 and utrophin are anchored to a novel membrane protein architecture, which consists of the SG and dystroglycan complexes, but not SPN, in the Schwann cell membrane.

+ To whom correspondence should be addressed. Tel: +81 423 46 1720; Fax: +81 423 46 1750; Email: imamura@ncnp.go.jp


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