Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (29)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Craig, A.
Right arrow Articles by Newbold, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Craig, A.
Right arrow Articles by Newbold, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Molecular Genetics, 2000, Vol. 9, No. 4 525-530
© 2000 Oxford University Press

A functional analysis of a natural variant of intercellular adhesion molecule-1 (ICAM-1Kilifi)

Alister Craig+, Delmiro Fernandez-Reyes, Mehdi Mesri1, Alison McDowall2, Dario C. Altieri1, Nancy Hogg2 and Christopher Newbold

Molecular Parasitology Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK, 1Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, PO Box 9812, New Haven, CT 06536-0812, USA and 2Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund Laboratories, PO Box123, Lincoln’s Inn Fields, London WC2A 3PX, UK

Intercellular adhesion molecule-1 (ICAM-1) is involved in a range of interactions both within the host and between the host and a number of pathogens. Recently we described a mutation within the coding region of the first N-terminal immunoglobulin-like domain of ICAM-1, present at high frequency within African populations, which increased the risk of cerebral malaria. To understand the mechanism by which such a polymorphism might be maintained despite counter-selection by malaria, we have carried out functional assays using both forms of ICAM-1 as soluble Fc chimeric fusion proteins. ICAM-1Kilifi has reduced avidity for LFA-1 compared with ICAM-1ref and binding to soluble fibrinogen was completely abolished with the Kilifi variant. In Plasmodium falciparum adhesion assays, ITO4-A4u binding to ICAM-1Kilifi was reduced compared with binding to the reference form. These results allow for the possibility of balanced selection between the reference and Kilifi forms of ICAM-1 through modulation of inflammatory responses and indicate the existence of differences within ICAM-1-binding P.falciparum isolates which may be relevant to pathogenesis.

+ To whom correspondence should be addressed. Tel: +44 151 708 9393; Fax: +44 151 708 9007; Email: agcraig@liverpool.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
B. C. Urban, D. Cordery, M. J. Shafi, P. C. Bull, C. I. Newbold, T. N. Williams, and K. Marsh
The Frequency of BDCA3-Positive Dendritic Cells Is Increased in the Peripheral Circulation of Kenyan Children with Severe Malaria
Infect. Immun., December 1, 2006; 74(12): 6700 - 6706.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. C. Register, K. P. Burdon, L. Lenchik, D. W. Bowden, G. A. Hawkins, B. J. Nicklas, K. Lohman, F.-C. Hsu, C. D. Langefeld, and J. J. Carr
Variability of Serum Soluble Intercellular Adhesion Molecule-1 Measurements Attributable to a Common Polymorphism
Clin. Chem., November 1, 2004; 50(11): 2185 - 2187.
[Full Text] [PDF]

Home page
 J. Virol.Home page
C. Xiao, T. J. Tuthill, C. M. Bator Kelly, L. J. Challinor, P. R. Chipman, R. A. Killington, D. J. Rowlands, A. Craig, and M. G. Rossmann
Discrimination among Rhinovirus Serotypes for a Variant ICAM-1 Receptor Molecule
J. Virol., September 15, 2004; 78(18): 10034 - 10044.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Fortin, M.M. Stevenson, and P. Gros
Susceptibility to malaria as a complex trait: big pressure from a tiny creature
Hum. Mol. Genet., October 1, 2002; 11(20): 2469 - 2478.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Gray and A. Craig
Fibrinogen Binding to Intercellular Adhesion Molecule 1: Implications for Plasmodium falciparum Adhesion
Infect. Immun., July 1, 2002; 70(7): 3962 - 3964.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
Alain. J. Dessein, C. Chevillard, S. Marquet, S. Henri, D. Hillaire, and H. Dessein
Genetics of Parasitic Infections
Drug Metab. Dispos., April 1, 2001; 29(4): 484 - 488.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.