Human Molecular Genetics, 2000, Vol. 9, No. 5 663-674
© 2000 Oxford University Press
The Schizosaccharomyces pombe protein Yab8p and a novel factor, Yip1p, share structural and functional similarity with the spinal muscular atrophy-associated proteins SMN and SIP1
Max-Planck-Institute for Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany and 1European Molecular Biology Laboratories, Meyerhofstraße 1, D-69117 Heidelberg, Germany
The motor neuron disease spinal muscular atrophy (SMA) is caused by reduced levels of functional survival of motor neurons (SMN) protein. Previous studies have shown that SMN binds to the SMN-interacting protein SIP1 and mediates the assembly of spliceosomal U snRNPs in the cytoplasm. In addition, a nuclear function for SMN in pre-mRNA splicing has recently been proposed. Here, we describe the analysis of the Schizosaccharomyces pombe protein Yab8p and provide evidence that it is structurally and functionally related to SMN found in higher eukaryotes. We show that Yab8p interacts via its N-terminus with a novel protein termed Yip1p. Importantly, Yip1p exhibits homology to SIP1, and the mode of binding to Yab8p is remarkably similar to the SMN–SIP1 interaction. Hence, Yip1p is likely to be the homologue of SIP1 in S.pombe. Yab8p and Yip1p localize predominantly in the nucleus. Genetic studies demonstrate that Yab8p is essential for viability. Strikingly, suppression of YAB8 expression in a conditional knock-out strain causes nuclear accumulation of poly(A) mRNA and inhibition of splicing. These data identify Yab8p as a novel factor involved in splicing and suggest that Yab8p exerts a function similar or identical to the nuclear pool of SMN. Our studies provide a model system to study the cellular function of SMN in yeast, and should help in understanding the molecular events leading to SMA.
+ To whom correspondence should be addressed. Tel: +49 89 8578 2475; Fax: +49 89 8578 3810; Email: ufischer@biochem.mpg.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. B. Shpargel, K. Praveen, T. K. Rajendra, and A. G. Matera Gemin3 Is an Essential Gene Required for Larval Motor Function and Pupation in Drosophila Mol. Biol. Cell, January 1, 2009; 20(1): 90 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kroiss, J. Schultz, J. Wiesner, A. Chari, A. Sickmann, and U. Fischer Evolution of an RNP assembly system: A minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster PNAS, July 22, 2008; 105(29): 10045 - 10050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Gonsalvez, K. Praveen, A. J. Hicks, L. Tian, and A. G. Matera Sm protein methylation is dispensable for snRNP assembly in Drosophila melanogaster RNA, May 1, 2008; 14(5): 878 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Carissimi, L. Saieva, J. Baccon, P. Chiarella, A. Maiolica, A. Sawyer, J. Rappsilber, and L. Pellizzoni Gemin8 Is a Novel Component of the Survival Motor Neuron Complex and Functions in Small Nuclear Ribonucleoprotein Assembly J. Biol. Chem., March 24, 2006; 281(12): 8126 - 8134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Feng, A. K. Gubitz, L. Wan, D. J. Battle, J. Dostie, T. J. Golembe, and G. Dreyfuss Gemins modulate the expression and activity of the SMN complex Hum. Mol. Genet., June 15, 2005; 14(12): 1605 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ilangovan, W. L. Marshall, Y. Hua, and J. Zhou Inhibition of Apoptosis by Z-VAD-fmk in SMN-depleted S2 Cells J. Biol. Chem., August 15, 2003; 278(33): 30993 - 30999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. B. Chan, I. Miguel-Aliaga, C. Franks, N. Thomas, B. Trulzsch, D. B. Sattelle, K. E. Davies, and M. van den Heuvel Neuromuscular defects in a Drosophila survival motor neuron gene mutant Hum. Mol. Genet., June 15, 2003; 12(12): 1367 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dez, J. Noaillac-Depeyre, M. Caizergues-Ferrer, and Y. Henry Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs Mol. Cell. Biol., October 15, 2002; 22(20): 7053 - 7065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baccon, L. Pellizzoni, J. Rappsilber, M. Mann, and G. Dreyfuss Identification and Characterization of Gemin7, a Novel Component of the Survival of Motor Neuron Complex J. Biol. Chem., August 23, 2002; 277(35): 31957 - 31962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jablonka, B. Holtmann, G. Meister, M. Bandilla, W. Rossoll, U. Fischer, and M. Sendtner Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death PNAS, July 23, 2002; 99(15): 10126 - 10131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Friesen, A. Wyce, S. Paushkin, L. Abel, J. Rappsilber, M. Mann, and G. Dreyfuss A Novel WD Repeat Protein Component of the Methylosome Binds Sm Proteins J. Biol. Chem., March 1, 2002; 277(10): 8243 - 8247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Voss, A. Hille, S. Barth, A. Spurk, F. Hennrich, D. Holzer, N. Mueller-Lantzsch, E. Kremmer, and F. A. Grasser Functional Cooperation of Epstein-Barr Virus Nuclear Antigen 2 and the Survival Motor Neuron Protein in Transactivation of the Viral LMP1 Promoter J. Virol., December 1, 2001; 75(23): 11781 - 11790. [Abstract] [Full Text]
|
|
|
|
|
|
K. W. Jones, K. Gorzynski, C. M. Hales, U. Fischer, F. Badbanchi, R. M. Terns, and M. P. Terns Direct Interaction of the Spinal Muscular Atrophy Disease Protein SMN with the Small Nucleolar RNA-associated Protein Fibrillarin J. Biol. Chem., October 12, 2001; 276(42): 38645 - 38651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pellizzoni, B. Charroux, J. Rappsilber, M. Mann, and G. Dreyfuss A Functional Interaction between the Survival Motor Neuron Complex and RNA Polymerase II J. Cell Biol., January 8, 2001; 152(1): 75 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. R. Monani, D. D. Coovert, and A. H.M. Burghes Animal models of spinal muscular atrophy Hum. Mol. Genet., October 1, 2000; 9(16): 2451 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Meister, D. Buhler, B. Laggerbauer, M. Zobawa, F. Lottspeich, and U. Fischer Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins Hum. Mol. Genet., August 12, 2000; 9(13): 1977 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Friesen and G. Dreyfuss Specific Sequences of the Sm and Sm-like (Lsm) Proteins Mediate Their Interaction with the Spinal Muscular Atrophy Disease Gene Product (SMN) J. Biol. Chem., August 18, 2000; 275(34): 26370 - 26375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang and G. Dreyfuss A Cell System with Targeted Disruption of the SMN Gene. FUNCTIONAL CONSERVATION OF THE SMN PROTEIN AND DEPENDENCE OF Gemin2 ON SMN J. Biol. Chem., March 23, 2001; 276(13): 9599 - 9605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang and G. Dreyfuss Characterization of Functional Domains of the SMN Protein in Vivo J. Biol. Chem., November 21, 2001; 276(48): 45387 - 45393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gubitz, Z. Mourelatos, L. Abel, J. Rappsilber, M. Mann, and G. Dreyfuss Gemin5, a Novel WD Repeat Protein Component of the SMN Complex That Binds Sm Proteins J. Biol. Chem., February 8, 2002; 277(7): 5631 - 5636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pellizzoni, J. Baccon, J. Rappsilber, M. Mann, and G. Dreyfuss Purification of Native Survival of Motor Neurons Complexes and Identification of Gemin6 as a Novel Component J. Biol. Chem., February 22, 2002; 277(9): 7540 - 7545. [Abstract] [Full Text] [PDF]
|
|