| ||||||||||||||||||||
SUPPLEMENTAL INFORMATION
a) VSX1 polymorphic nucleotide changes
|
Exon |
Change |
|
|
Exon1 |
Ser6Ser |
tcg-tct |
|
|
|
|
|
Exon3 |
Ala182ala |
Gca-gcg |
|
|
|
|
|
Exon 4 |
g-a 23bp3’end arg215arg |
918c-g |
|
|
|
|
|
Exon 5 |
1bp del 6bp 5’end |
|
|
|
|
|
According to Acc#AF251033
b) Primers used for amplification of the coding sequence of VSX1
|
Exon |
Primer Name |
Primer sequence (5'-3') |
|
1a |
VSX 1
rc 1af |
atggtctgtgacccctgc |
|
1b |
VSX 1
rc 1br |
ttcgccatcacggacctgc |
|
1b |
VSX 1
rc 1bf |
gaacggcacgtccgctag |
|
1c |
VSX 1
rc 1cr |
ctataaagcttcctctaagc |
|
1a |
VSX 1
rc 1ar |
tgtggcttcggcacgcag |
|
1c |
VSX 1
rc 1cf |
agccagatccctgtcctg |
|
2 |
VSX 1 2
for |
cattttcccagcctccta |
|
2 |
VSX 1 2
rev |
ttaagtacccaagaggttca |
|
3 |
VSX 1
rc 3f |
gacacgttctctgtggtatc |
|
3 |
VSX 1
rc 3r |
acagcagaggaagcaggcac |
|
4 |
VSX 1
rc 4f |
tcctacaacacctcgagc |
|
4 |
VSX 1
rc 4r |
ctcttcactgtctcttctg |
|
5a |
VSX 1
rc 5bf |
agctggagaggtcaatagc |
|
5a |
VSX 1
rc 5br |
agataggcactgacaaggac |
|
5b |
VSX 1
5afor |
ggcattgcattttatcttgac |
|
5b |
VSX 1
5arev |
cttcaaagaaggttctagcc |
|
|
|
|
|
The
forward primers are sense strand and reverse primers-antisense. |
||
|
All
forward primers have an M13 tail (5'-gtaaaacgacggccagt-3') on the 5' end and |
||
|
reverse primers have and M13 reverse tail
(5'-cacaggaaacagctatgac-3') |
||
|
|
||
| ||||||||||||||||||||