Human Molecular Genetics

This Article Abstract FREE Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Archive Download to citation manager Search for citing articles in: ISI Web of Science (56) Request Permissions Google Scholar Articles by Jacobson, S. G. Articles by Stone, E. M. Search for Related Content PubMed PubMed Citation Articles by Jacobson, S. G. Articles by Stone, E. M. Social Bookmarking          What's this?

Human Molecular Genetics, 2003, Vol. 12, No. 9 1073-1078
DOI: 10.1093/hmg/ddg117
© 2003 Oxford University Press

Crumbs homolog 1 (CRB1) mutations result in a thick human retina with abnormal lamination

Samuel G. Jacobson^1,*, Artur V. Cideciyan¹, Tomas S. Aleman¹, Michael J. Pianta¹, Alexander Sumaroka¹, Sharon B. Schwartz¹, Elaine E. Smilko¹, Ann H. Milam¹, Val C. Sheffield^2,4 and Edwin M. Stone^3,4

¹Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104, USA and ²Department of Pediatrics, ³Department of Ophthalmology and ⁴Howard Hughes Medical Institute, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA

Received February 3, 2003; Revised February 24, 2003; Accepted March 2, 2003

	ABSTRACT

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 
Mutations in CRB1, the human homolog of Drosophila Crumbs, cause autosomal recessive blinding disorders of the retina. Whereas Crumbs is implicated in apical-basal epithelial polarity and photoreceptor morphogenesis, the role of CRB1 in normal or diseased retina remains unclear. We characterized the retinal organization in vivo of patients with CRB1 mutations and found that, unlike other inherited retinal degenerations studied to date, the CRB1 mutant retinas are remarkably thick in cross-section and lack the distinct layers of normal adult retina. There are coarse outer and inner zones and a thick surface layer around the optic nerve. The abnormal retinal architecture in CRB1 mutations resembles that of immature normal retina. The results suggest that the CRB1 disease pathway disturbs the development of normal human retinal organization by interrupting naturally occurring apoptosis.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 
Crumbs (Crb) was the first Drosophila gene shown to be a key developmental regulator of epithelial apical-basal polarity (1). The transmembrane protein Crb is now known to be one of many protein complexes along the plasma membrane that participate in cell-to-cell contacts, such as zonulae adherentes. The molecular interactions of Crb with other proteins controlling epithelial cell polarity in Drosophila are actively being explored (2,3). CRB1, one of three Crb human homologues (4), came to scientific and medical attention when mutations in this gene were identified in severe forms of retinal blindness known as retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA) (5–7).

The sequence from CRB1 mutation to the human phenotype of congenital retinal blindness seemed more understandable when a role in retinal photoreceptor morphogenesis was demonstrated for Drosophila Crb and murine Crb1 (8,9). CRB1 would be expected to be important for human photoreceptor function and structure (8,9) and Crb and CRB1 were suggested to have conserved functions in eye development (4). Mutations in CRB1 certainly lead to loss of photoreceptor signaling by clinical electrophysiological testing in affected patients (7), but so do all other known genetic causes of RP and LCA (10). Little further detail about the pathogenesis of human CRB1 mutation has been forthcoming and there is currently no mammalian disease model to gain insight into the detailed mechanism.

Here we used in vivo high-resolution microscopy (11) to study the retinas of patients with CRB1 mutations. In contrast to the phenotypes of other inherited retinal degenerations studied to date by histopathology (12) or in vivo microscopy (13,14), CRB1 mutant retinas were abnormally thick rather than thinned and the lamination pattern was coarse, resembling certain stages of normal human retinal development (15–17). These unexpected results in CRB1 mutations may be due in part to a disturbance in retinal histogenesis with interrupted naturally occurring apoptosis.

	RESULTS

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 
The CRB1 mutant human retina viewed en face (Fig. 1A, inset) appears abnormal due to pigment clumping (18). This surface view cannot define pathological features in depth because the retina is transparent. We thus used high-resolution cross-sectional retinal imaging, known as optical coherence tomography (OCT) (11,19–21), to study the in vivo micropathology of LCA due to CRB1 mutations and compared the results to LCA from other molecular causes (10).

View larger version (44K): [in this window] [in a new window]   Figure 1. CRB1 mutant retina, en face and cross-sectional views. (A) Cross-sectional image from the central retina of a 25-year-old normal subject and a 26-year-old patient with CRB1 mutation. Vitreous is above. Insets are fundus views; arrowheads point to the location of the cross-section. (B) Retinal thickness in normal subjects (gray band=mean±2 SD) compared with patients having CRB1 (black traces), RPE65 (green traces) or GUCY2D (red trace) mutations. Different line types and thicknesses are used for individual patients to permit comparisons; numbers shown to the left of each trace correspond to the patient numbers in Table 1.

 
Normal human central retina viewed with OCT has a foveal depression and discernible layers (Fig. 1A, upper part). A CRB1 mutant retina (Table 1; patient 6) also has a foveal depression, but the surrounding retina appears thicker than normal with less distinct lamination (Fig. 1A, lower part). We quantified central retinal thickness (Fig. 1B) in eight CRB1-LCA patients (ages 2–50; Table 1) and related these data to normal (n=18; ages 4–29). CRB1 mutant retinas have a foveal depression but the adjacent retina is abnormally thick. To determine if this result is CRB1-specific, we analysed retinal thickness in LCA due to mutations in RPE65, a retinal pigment epithelium (RPE) gene involved in retinoid metabolism, and GUCY2D, a photoreceptor gene controlling phototransduction recovery (Table 1; patients 9–11). These patients have normal or reduced retinal thickness (Fig. 1B), like other retinal degenerations (12–14).

View this table: [in this window] [in a new window]   Table 1. Mutations in patients with LCA

 
When we examined the laminar organization of CRB1 mutant retinas, we found that the thick retinas were not simply expanded versions of normal. Normal human retinal lamination is shown in a histological section from the temporal retina of a 53-year-old man (Fig. 2A, left). There are three cellular layers (ganglion cell layer; inner nuclear layer; outer nuclear layer); two synaptic layers (inner and outer plexiform layers); the inner limiting membrane (the basal lamina of Muller glia); photoreceptor inner and outer segments; the outer limiting membrane (zonulae adherentes that join photoreceptor inner segments to Muller glia); and the RPE. The nerve fiber layer is not visible at this locus along the horizontal raphe. An OCT retinal cross-section from a normal 58-year-old man is shown at the same temporal location (Fig. 2A, right). Bands of low and high reflectivity are discernible: cellular layers generally correspond to low reflectivity OCT bands and synaptic layers have higher reflectivity. Photoreceptor, RPE and anterior choroid form the highly reflective layer deep in the retina (20,21).

View larger version (61K): [in this window] [in a new window]   Figure 2. Laminar organization of the CRB1 mutant retina. (A) Retinal histology 3 mm temporal to the fovea from a 53-year-old normal subject (cell nuclei stained blue) compared with a cross-sectional retinal image in gray scale from a normal 58-year-old. Ganglion cell layer (GCL); inner (INL) and outer (ONL) nuclear layers; inner (IPL) and outer (OPL) plexiform layers; inner (ILM) and outer (OLM) limiting membranes; photoreceptor (PR) inner (IS) and outer (OS) segments; retinal pigment epithelium (RPE); and choroid (CH) are shown. The calibration bar applies to A and B. (B) Reflectivity waveforms in normal subjects and three LCA genotypes also at 3 mm temporal to the fovea. Black bands underlying the waveforms suggest locations of INL and ONL. The average CRB1 reflectivity waveform (thick dark line) is overlaid on five individual waveforms (thin lines). Labeled brackets indicate three zones that differ in reflectivity and depth. (C) Comparison of average reflectivity waveforms at temporal (thicker lines) and nasal (thin lines) retinal locations in normal and CRB1 mutant retinas. The blue bar (left) indicates nerve fiber layer measurement at a comparable nasal locus in normal cadaver eyes (from 22) and serves also as a calibration for the retinal depth axis of the reflectivity waveforms. (D) Spatial distribution of the nerve fiber layer within an annular region (inset, between white circles) around the optic nerve (ON) of a CRB1 mutant retina (upper) compared with average normal (lower). (E) Polar plot of nerve fiber layer thickness at a circle (diameter, 3.4 mm) around optic nerve (inset, black circle) in normal subjects (gray band=mean±2 SD) and five individuals with CRB1 mutations (black traces). Blue symbols indicate NFL measurements from cadaver eyes (from 22) at comparable loci along the OCT scan.

 
Reflectivity waveforms, which comprise the OCT scans, show the differences in retinal layering between normals and LCA in temporal retina (Fig. 2B) and nasal to the fovea near the optic nerve (Fig. 2C). An average normal (n=3, ages 19–25) waveform from temporal retina (Fig. 2B) has peaks and troughs corresponding to the light and dark bands in Figure 2A. RPE65 and GUCY2D mutant retinas are thinned mainly due to loss of outer nuclear layer and photoreceptor inner and outer segment components. These waveforms resemble those in other human and animal retinal degenerations with photoreceptor loss (13,14,20,21). In contrast, the reflectivity waveforms from CRB1 mutant retinas are about 1.5 times thicker than normal (Figs 1B and 2B) and the pattern does not relate easily to normal layering. We defined three reflectivity zones: ß, an inner zone of high reflectivity; , an adjacent deeper zone with lower reflectivity; and , the deepest zone which, like the normal photoreceptor-RPE-choroidal complex, is highest in reflectivity.

Nerve fiber layer thickness normally increases toward the optic nerve as ganglion cell axons from across the retina converge to exit the eye. By OCT, the nerve fiber layer is a band of high reflectivity near the retinal surface (Fig. 1A). Overlaid reflectivity waveforms from nasal and temporal retina in normal subjects illustrate the additional surface reflection (Fig. 2C, left), which relates well to nerve fiber layer thickness by histology (22). The CRB1 mutant retina also has an additional surface reflection present nasally but not temporally in overlaid waveform templates (Fig. 2C, right). This component in CRB1, labeled , is far thicker than normal. The spatial distribution of (Fig. 2D) within an annular region around the optic nerve (inset) in a CRB1 mutant retina (Table 1; patient 3), compared with normal nerve fiber layer (n=4; ages 19–25), illustrates the pronounced thickening in CRB1. Polar plots of nerve fiber layer thickness at a single radius (Fig. 2E) around the optic nerve (inset) in five CRB1 eyes (patients 2–6) confirm that the LCA inner retinal layer () is indeed thicker than normal nerve fiber layer (n=24; ages 8–41). Histological nerve fiber layer measurements at four loci in normal human eyes (22) fall within our normal range by OCT measurement (Fig. 2E).

	DISCUSSION

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 
This is the first report of coarsely-laminated thick retinas in LCA or any human genetic retinal disease. The opposite, retinal thinning, is usually observed and was described recently by histopathology in LCA due to RPE65 (23) and GUCY2D (24) mutations, consistent with our in vivo results in this study. Normal human fetal retina, however, is thick and coarsely laminated (15–17). For example, at about 24 weeks of gestation, there can be a foveal depression but only ‘primordial’ lamination in more peripheral retina, 1.5-fold numbers of ganglion cells and 2-fold optic axon numbers, compared with the adult (15–17,25,26). Also, thick retinas with increased neurons and synapses are features of mice with disturbed naturally occurring apoptosis during development, such as bcl-2 overexpressing transgenic and bax-/-mice (reviewed in 27).

We hypothesize that the CRB1 mutant retinal phenotype represents an immature lamination pattern resulting from interrupted naturally occurring developmental apoptosis. The broad inner retinal zone (ß) would be a layer with increased ganglion cells among synaptic and glial elements. The higher reflectivity () around the optic nerve would represent excess ganglion cell axons (16,17). The deeper low reflectivity () would be an undeveloped nuclear zone (16,17) and the deepest high reflection () and backscatter would be abnormal photoreceptor/RPE layers, possibly with photoreceptor rosettes.

Understanding the causal pathways leading to this previously unrecognized retinal phenotype in LCA will require considerable further work on the precise role of CRB1 during human retinal development. Murine Crb1 is considered to be important for development of photoreceptor cells, most notably in zonula adherens assembly (4,8,9). Zonulae adherentes form the outer limiting membrane, the site of interconnection of photoreceptor inner segments and Muller glia. Dysplasia mainly at the photoreceptor level can result from outer limiting membrane disruption and Muller glia abnormalities (28,29), but the type of lamination defects that extend from outer to innermost retina, such as we found in CRB1 mutations, has not been described. It would be intriguing if the abnormal CRB1 retinal organization is the consequence of perturbed communication from neuron-to-glia-to-neuron (specifically ganglion cells, the radially-oriented Muller glia and photoreceptors), a signaling pathway proving to be important in vertebrate and invertebrate retinal laminar development (30). From a clinical perspective, it will be valuable to determine how these structural defects affect retinal circuitry and possibly central visual pathways. We could then assess the relevance to patients with CRB1 mutations of therapeutic strategies emerging for other LCA genotypes (31).

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 
Subjects: clinical and molecular analyses
Informed consent for all procedures was obtained from subjects after the nature of the studies had been explained. Research procedures were in accordance with institutional guidelines and the tenets of the Declaration of Helsinki. Blood samples were obtained and DNA extracted (32). Probands were screened for mutations in CRB1, RPE65 and GUCY2D using single-strand conformation polymorphism analysis (SSCP). The primer sequences used have been reported (5,7,33,34). The polymerase chain reaction (PCR) amplification products were denatured for 3 min at 94°C and then electrophoresed on 6% polyacrylamide–5% glycerol gels at 25 W for about 3 h. The gels were stained with silver nitrate (35). PCR products showing shifts were sequenced bidirectionally using an automated sequencer (model 377; Applied Biosystems, Foster City, CA, USA).

Table 1 shows participating patients (n=11) and their gene mutations. Subjects with normal eye examinations (n=26; ages 4–58 years) were included as controls for the various studies. All patients had severe retinopathy that falls within the spectrum of LCA. Two of the eight patients with CRB1 mutations (Table 1; patients 3 and 4), however, showed better visual acuity (20/40 and 20/60, respectively) than the others (range, 20/100 to light perception). An alternative diagnosis for these two patients could be early-onset severe RP. The patterns of inheritance in the CRB1 patient group were autosomal recessive (n=1), multiplex (n=3) and simplex (n=4). Most of the patients were hyperopic and had nystagmus. All CRB1 patients had limited kinetic visual fields, mainly showing severely impaired central and far peripheral islands only. Electroretinography indicated no detectable responses to standard stimuli except in patients 1 and 3, who had measurable but abnormally reduced waveform amplitudes. Patient 8 had keratoconus and had undergone bilateral corneal transplants years before his visit. Most patients had pigmentary abnormalities across the fundus and attenuated retinal vessels. Optic nerve drusen were not present. Three patients showed preserved para-arteriolar retinal pigment epithelium and one had Coat's disease (18,36).

Optical coherence tomography: data acquisition
Cross-sectional retinal reflectivity profiles were obtained with optical coherence tomography (OCT; Zeiss Humphrey Instruments, Dublin, CA, USA). The principles of the method (19,37) and our techniques (13,14,20,21) have been published. For the current work, data were acquired on two types of instruments: a first-generation OCT1 with a theoretical axial resolution in retinal tissue of 10 µm and a third-generation OCT3 with a resolution of 8 µm. All patients and all normal subjects were scanned with OCT1; in addition, six patients with CRB1 mutations as well as a subset of normal subjects were scanned with OCT3. Three types of scan groups were used to sample the retinal cross-section across the posterior pole. One scan group consisted of five overlapping segments of linear scans, each of 4.5 mm length, located along the horizontal meridian, centered on the anatomical fovea and extending to 9 mm in either direction. The second scan group consisted of six linear scans, each of 4 mm length, in a regular spoke pattern (with 30° increments) centered on the optic nerve. The third group was three concentric circles of 1.8, 3.6 and 5.4 mm diameter, also centered on the optic nerve. In addition, a circular scan of 3.4 mm diameter and a horizontal linear scan of 4.5 mm length were acquired centered on the optic nerve. Each type and location of scan was repeated at least twice and usually many times. A video fundus image was acquired and saved with each OCT scan by the commercial software. In addition, the fundus video visible during the complete session was recorded continuously on a video cassette recorder. This allowed post-acquisition analysis of possible eye movements occurring during a scan (see below).

Many of the patients displayed involuntary eye movements (nystagmus) which complicated the recording of OCT scans. In most cases, the saccadic amplitudes of the nystagmus varied over time during the session. To obtain OCT scans, we waited for near-steady periods, judged by looking at the real-time fundus view. In cases with more severe nystagmus, video records from the session were analysed post-acquisition and data from scans obtained during saccades were discarded. This accounts for partial retinal thickness data displayed in Figure 1B for three of eight patients with CRB1 mutation and the patient with GUCY2D mutation.

OCT: data processing and quantification
Post-acquisition processing of OCT data was mostly performed with custom programs (MATLAB 6.5, MathWorks, Natick, MA, USA). OCT cross-sectional data are presented either on a pseudocolor scale (see bar in Fig. 1A) or a gray scale; displays have the vitreous above. For magnified images (see right panel in Fig. 2A), a 5x1 moving median filter was used to smooth speckle noise in lateral dimension while keeping the original high resolution in the longitudinal direction. For quantitative analysis, the precise location and orientation of each scan relative to retinal features (blood vessels and optic nerve head) were determined using the video images of the fundus. The individual reflectivity waveforms (Fig. 2B and C) were allotted to 0.3 mm bins in a rectangular coordinate system centered at the fovea; the waveforms in each bin were aligned using a dynamic cross-correlation algorithm (20,21), normalized and averaged. The retinal thickness, as measured by OCT, was assumed to be the distance between features T1 (corresponding to the vitreo-retinal interface) and T5 (corresponding to the maximal slope sclerad to the signal maximum, as previously defined in 20,21,38). Reflectivity waveform templates from a group of individuals (Fig. 2B and C) were produced by first scaling each waveform to the average T1–T5 distance of the respective group and then averaging the scaled waveforms. This preserved both quantitative thickness measures as well as waveform shape.

Thickness of the nerve fiber layer was calculated with the commercial programs included in each OCT instrument. In short, the commercial software uses the depth of the high reflectivity signal near the vitreo-retinal interface as an accepted measure of the thickness of nerve fiber layer around the optic nerve region (11,37,39). For each of the 12 scans around the optic nerve (six concentric scans, six linear scans in a spoke pattern), nerve fiber layer thickness was determined as a function of scan position. A custom program (MATLAB 6.5) was used to sample (using bilinear interpolation) the non-uniformly spaced nerve fiber layer thickness data from these 12 scans onto a uniform (30 µm) square grid in a 6x6 mm region centered at the optic nerve. The resulting data were spatially filtered with a moving average filter (300x300 µm in extent) five times to smooth out noise, mapped to a pseudocolor scale and presented as a floating ‘surface’ illuminated by two point light sources. In addition, the nerve fiber layer thickness determined from the 3.4 mm diameter ‘standard’ circular scans was presented as a polar plot where the location around the optic nerve defined the angle and the thickness defined the radial length of each datum. All data were displayed as equivalent right eyes.

Retinal histology
An eye from a 53-year-old man with normal vision was obtained after surgical exenteration for an orbital tumor. The eye was opened and fixed immediately in 4% paraformaldehyde in 0.1 M phosphate buffer. The central retina containing the macula and optic nerve head was dissected and infiltrated overnight in 30% sucrose in the same buffer at 4°C. The tissue was cryosectioned at 12 µm thickness and processed for immunofluorescence with a cone specific antibody (mAb 7G6, 1:250, from Dr P. MacLeish, Morehouse School of Medicine, Atlanta, GA, USA). Secondary anti-mouse IgG (1:50) was labeled (red) with Cy-3 (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). Cell nuclei were stained (blue) with 4',6'-diamidino-2-phenylindole (1 µg/ml; Molecular Probes, Eugene, OR, USA). Control sections were treated in the same way with omission of primary antibody. The immunolabeled retinal sections were imaged with a microscope equipped for epifluorescence (Leica DMR, Deerfield, IL, USA).

	ACKNOWLEDGEMENTS

 
We thank J. Emmons, M. Kulon, Y. Huang, I. Knizhnik, J. Chico, A. Cheung, M. Nguyen, K. Przychodzen, E. Windsor, A. Pantelyat, J. Smith and M. Swider for critical help. V.C.S. is an associate investigator and E.M.S. is an investigator of the Howard Hughes Medical Institute. This work was supported by the National Institutes of Health EY13385, EY13729, EY13203, Foundation Fighting Blindness, Daniel Matzkin Research Fund and Macula Vision Research Foundation.

	FOOTNOTES

 
^* To whom correspondence should be addressed at: Scheie Eye Institute, University of Pennsylvania, 51 N. 39th Street, Philadelphia, PA 19104, USA. Tel: +1 2156629981; Fax: +1 2156629388; Email: jacobsos{at}mail.med.upenn.edu

	REFERENCES

TOP ABSTRACT INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS REFERENCES

 

1. Tepass, U., Theres, C. and Knust, E. (1990) crumbs encodes an EGF-like protein expressed on apical membranes of Drosophila epithelial cells and required for organization of epithelia. Cell, 61, 787–799.[CrossRef][Web of Science][Medline]

2. Bilder, D., Schober, M. and Perrimon, N. (2003) Integrated activity of PDZ protein complexes regulates epithelial polarity. Nat. Cell Biol., 5, 53–58.[CrossRef][Web of Science][Medline]

3. Tanentzapf, G. and Tepass, U. (2003) Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization. Nat. Cell Biol., 5, 46–52.[CrossRef][Web of Science][Medline]

4. Tepass, U. (2002) Adherens junctions: new insight into assembly, modulation and function. Bioessays, 24, 690–695.[CrossRef][Web of Science][Medline]

5. den Hollander, A.I., ten Brink, J.B., de Kok, Y.J., van Soest, S., van den Born, L.I., van Driel, M.A., van de Pol, D.J., Payne, A.M., Bhattacharya, S.S., Kellner, U. et al. (1999) Mutations in a human homologue of Drosophila crumbs cause retinitis pigmentosa (RP12). Nat. Genet., 23, 217–221.[CrossRef][Web of Science][Medline]

6. den Hollander, A.I., Heckenlively, J.R., van den Born, L.I., de Kok, Y.J., van der Velde-Visser, S.D., Kellner, U., Jurklies, B., van Schooneveld, M.J., Blankenagel, A., Rohrschneider, K. et al. (2001) Leber congenital amaurosis and retinitis pigmentosa with Coats-like exudative vasculopathy are associated with mutations in the crumbs homologue 1 (CRB1) gene. Am. J. Hum. Genet., 69, 198–203.[CrossRef][Web of Science][Medline]

7. Lotery, A.J., Jacobson, S.G., Fishman, G.A., Weleber, R.G., Fulton, A.B., Namperumalsamy, P., Heon, E., Levin, A.V., Grover, S., Rosenow, J.R. et al. (2001) Mutations in the CRB1 gene cause Leber congenital amaurosis. Arch. Ophthal., 119, 415–420.[Abstract/Free Full Text]

8. Pellikka, M., Tanentzapf, G., Pinto, M., Smith, C., McGlade, C.J., Ready, D.F. and Tepass, U. (2002) Crumbs, the Drosophila homologue of human CRB1/RP12, is essential for photoreceptor morphogenesis. Nature, 416, 143–149.[CrossRef][Medline]

9. Izaddoost, S., Nam, S.C., Bhat, M.A., Bellen, H.J. and Choi, K.W. (2002) Drosophila Crumbs is a positional cue in photoreceptor adherens junctions and rhabdomeres. Nature, 416, 178–183.[CrossRef][Medline]

10. Cremers, F.P., van den Hurk, J.A. and den Hollander, A.I. (2002) Molecular genetics of Leber congenital amaurosis. Hum. Mol. Genet., 11, 1169–1176.[Abstract/Free Full Text]

11. Drexler, W., Morgner, U., Ghanta, R.K., Kartner, F.X., Schuman, J.S. and Fujimoto, J.G. (2001) Ultrahigh-resolution ophthalmic optical coherence tomography. Nat. Med., 7, 502–507.[CrossRef][Web of Science][Medline]

12. Milam, A.H., Li, Z.Y. and Fariss, R.N. (1998) Histopathology of the human retina in retinitis pigmentosa. Prog. Retin. Eye Res., 17, 175–205.[CrossRef][Web of Science][Medline]

13. Jacobson, S.G., Cideciyan, A.V., Huang, Y., Hanna, D.B., Freund, C.L., Affatigato, L.M., Carr, R.E., Zack, D.J., Stone, E.M. and McInnes, R.R. (1998) Retinal degenerations with truncation mutations in the cone-rod homeobox (CRX) gene. Invest. Ophthal. Visual Sci., 39, 2417–2426.[Abstract/Free Full Text]

14. Jacobson, S.G., Cideciyan, A.V., Iannaccone, A., Weleber, R.G., Fishman, G.A., Maguire, A.M., Affatigato, L.M., Bennett, J., Pierce, E.A., Danciger, M. et al. (2000) Disease expression of RP1 mutations causing autosomal dominant retinitis pigmentosa. Invest. Ophthal. Visual Sci., 41, 1898–1908.[Abstract/Free Full Text]

15. Mann, I. (1949) The Development of the Human Eye. British Medical Association, London.

16. Provis, J.M., van Driel, D., Billson, F.A. and Russell, P. (1985) Development of the human retina: patterns of cell distribution and redistribution in the ganglion cell layer. J. Comp. Neurol., 233, 429–451.[CrossRef][Web of Science][Medline]

17. Provis, J.M., van Driel, D., Billson, F.A. and Russell, P. (1985) Human fetal optic nerve: overproduction and elimination of retinal axons during development. J. Comp. Neurol., 238, 92–100.[CrossRef][Web of Science][Medline]

18. Heckenlively, J.R. (1982) Preserved para-arteriole retinal pigment epithelium (PPRPE) in retinitis pigmentosa. Br. J. Ophthal., 66, 26–30.[Abstract/Free Full Text]

19. Huang, D., Swanson, E.A., Lin, C.P., Schuman, J.S., Stinson, W.G., Chang, W., Hee, M.R., Flotte, T., Gregory, K., Puliafito, C.A. et al. (1991) Optical coherence tomography. Science, 254, 1178–1181.[Abstract/Free Full Text]

20. Huang, Y., Cideciyan, A.V., Papastergiou, G.I., Banin, E., Semple-Rowland, S.L., Milam, A.H. and Jacobson, S.G. (1998) Relation of optical coherence tomography to microanatomy in normal and rd chickens. Invest. Ophthal. Visual Sci., 39, 2405–2416.[Abstract/Free Full Text]

21. Huang, Y., Cideciyan, A.V., Aleman, T.S., Banin, E., Huang, J., Syed, N.A., Petters, R.M., Wong, F., Milam, A.H. and Jacobson, S.G. (2000) Optical coherence tomography (OCT) abnormalities in rhodopsin mutant transgenic swine with retinal degeneration. Exp. Eye Res., 70, 247–251.[CrossRef][Web of Science][Medline]

22. Varma, R., Skaf, M. and Barron, E. (1996) Retinal nerve fiber layer thickness in normal human eyes. Ophthalmology, 103, 2114–2119.[Web of Science][Medline]

23. Porto, F.B., Perrault, I., Hicks, D., Rozet, J.M., Hanoteau, N., Hanein, S., Kaplan, J. and Sahel, J.A. (2002) Prenatal human ocular degeneration occurs in Leber's congenital amaurosis (LCA2). J. Gene Med., 4, 390–396.[CrossRef][Web of Science][Medline]

24. Milam, A.H., Barakat, M.R., Gupta, N., Rose, L., Aleman, T.S., Pianta, M.J., Cideciyan, A.V., Sheffield, V.C., Stone, E.M. and Jacobson, S.G. (2003) Clinicopathologic effects of mutant GUCY2D in Leber congenital amaurosis. Ophthalmology, 110, 549--558

25. Hendrickson, A.E. (1994) Primate foveal development: a microcosm of current questions in neurobiology. Invest. Ophthal. Visual Sci., 35, 3129–3133.[Free Full Text]

26. Provis, J.M., Diaz, C.M. and Dreher, B. (1998) Ontogeny of the primate fovea: a central issue in retinal development. Prog. Neurobiol., 54, 549–580.[CrossRef][Web of Science][Medline]

27. Cellerino, A., Bahr, M. and Isenmann, S. (2000) Apoptosis in the developing visual system. Cell Tissue Res., 301, 53–69.[CrossRef][Web of Science][Medline]

28. Dyer, M.A. and Cepko, C.L. (2000) Control of Müller glial cell proliferation and activation following retinal injury. Nat. Neurosci., 3, 873–880.[CrossRef][Web of Science][Medline]

29. Willbold, E., Rothermel, A., Tomlinson, S. and Layer, P.G. (2000) Müller glia cells reorganize reaggregating chicken retinal cells into correctly laminated in vitro retinae. Glia, 29, 45–57.[CrossRef][Web of Science][Medline]

30. Wang, Y.P., Dakubo, G., Howley, P., Campsall, K.D., Mazarolle, C.J., Shiga, S.A., Lewis, P.M., McMahon, A.P. and Wallace, V.A. (2002) Development of normal retinal organization depends on Sonic hedgehog signaling from ganglion cells. Nat. Neurosci., 5, 831–832.[Web of Science][Medline]

31. Acland, G.M., Aguirre, G.D., Ray, J., Zhang, Q., Aleman, T.S., Cideciyan, A.V., Pearce-Kelling, S.E., Anand, V., Zeng, Y., Maguire, A.M. et al. (2001) Gene therapy restores vision in a canine model of childhood blindness. Nat. Genet., 28, 92–95.[CrossRef][Web of Science][Medline]

32. Buffone, G.J. and Darlington, G.J. (1985) Isolation of DNA from biological specimens without extraction with phenol. Clin. Chem., 31, 164–165.[Free Full Text]

33. Perrault, I., Rozet, J.M., Calvas, P., Gerber, S., Camuzat, A., Dollfus, H., Chatelin, S., Souied, E., Ghazi, I., Leowski, C. et al. (1996) Retinal-specific guanylate cyclase gene mutations in Leber's congenital amaurosis. Nat. Genet., 14, 461–464.[CrossRef][Web of Science][Medline]

34. Marlhens, F., Bareil, C., Griffoin, J.M., Zrenner, E., Amalric, P., Eliaou, C., Liu, S.Y., Harris, E., Redmond, T.M., Arnaud, B. et al. (1997) Mutations in RPE65 cause Leber's congenital amaurosis. Nat. Genet., 17, 139–141.[CrossRef][Web of Science][Medline]

35. Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P.M. (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem., 196, 80–83.[CrossRef][Web of Science][Medline]

36. van den Born, L.I., van Soest, S., van Schooneveld, M.J., Riemslag, F.C., de Jong, P.T. and Bleeker-Wagemakers, E.M. (1994) Autosomal recessive retinitis pigmentosa with preserved para-arteriolar retinal pigment epithelium. Am. J. Ophthal., 118, 430–439.[Web of Science][Medline]

37. Hee, M.R., Izatt, J.A., Swanson, E.A., Huang, D., Schuman, J.S., Lin, C.P., Puliafito, C.A. and Fujimoto, J.G. (1995) Optical coherence tomography of the human retina. Arch. Ophthal., 113, 325–332.[Abstract/Free Full Text]

38. Kijas, J.W., Cideciyan, A.V., Aleman, T.S., Pianta, M.J., Pearce-Kelling, S.E., Miller, B.J., Jacobson, S.G., Aguirre, G.D. and Acland, G.M. (2002) Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa. Proc. Natl Acad. Sci. USA, 99, 6328–6333.[Abstract/Free Full Text]

39. Schuman, J.S. (1996) Imaging optic nerve fiber layer: Optical coherence tomography. In Bucci, M.G. (ed.), Glaucoma: Decision Making in Therapy. Springer, New York, NY, pp. 47–50.

40. Lotery, A.J., Namperumalsamy, P., Jacobson, S.G., Weleber, R.G., Fishman, G.A., Musarella, M.A., Hoyt, C.S., Heon, E., Levin, A., Jan, J. et al. (2000) Mutation analysis of 3 genes in patients with Leber congenital amaurosis. Arch. Ophthal., 118, 538–543.[Abstract/Free Full Text]

41. Thompson, D.A., Gyurus, P., Fleischer, L.L., Bingham, E.L., McHenry, C.L., Apfelstedt-Sylla, E., Zrenner, E., Lorenz, B., Richards, J.E., Jacobson, S.G. et al. (2000) Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration. Invest. Ophthal. Visual Sci., 41, 4293–4299.[Abstract/Free Full Text]

42. Lorenz, B., Gyurus, P., Preising, M., Bremser, D., Gu, S., Andrassi, M., Gerth, C. and Gal, A. (2000) Early-onset severe rod-cone dystrophy in young children with RPE65 mutations. Invest. Ophthal. Visual Sci., 41, 2735–2742.

CiteULike    Connotea    Del.icio.us    What's this?

This article has been cited by other articles:

				T. Maeda, A. V. Cideciyan, A. Maeda, M. Golczak, T. S. Aleman, S. G. Jacobson, and K. Palczewski Loss of cone photoreceptors caused by chromophore depletion is partially prevented by the artificial chromophore pro-drug, 9-cis-retinyl acetate Hum. Mol. Genet., June 15, 2009; 18(12): 2277 - 2287. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, T. S. Aleman, A. V. Cideciyan, A. J. Roman, A. Sumaroka, E. A. M. Windsor, S. B. Schwartz, E. Heon, and E. M. Stone Defining the Residual Vision in Leber Congenital Amaurosis Caused by RPE65 Mutations Invest. Ophthalmol. Vis. Sci., May 1, 2009; 50(5): 2368 - 2375. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, T. S. Aleman, A. Sumaroka, A. V. Cideciyan, A. J. Roman, E. A. M. Windsor, S. B. Schwartz, H. L. Rehm, and W. J. Kimberling Disease Boundaries in the Retina of Patients with Usher Syndrome Caused by MYO7A Gene Mutations Invest. Ophthalmol. Vis. Sci., April 1, 2009; 50(4): 1886 - 1894. [Abstract] [Full Text] [PDF]

				C. J. Abbott, N. A. McBrien, U. Grunert, and M. J. Pianta Relationship of the Optical Coherence Tomography Signal to Underlying Retinal Histology in the Tree Shrew (Tupaia belangeri) Invest. Ophthalmol. Vis. Sci., January 1, 2009; 50(1): 414 - 423. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, A. V. Cideciyan, T. S. Aleman, A. Sumaroka, E. A. M. Windsor, S. B. Schwartz, E. Heon, and E. M. Stone Photoreceptor Layer Topography in Children with Leber Congenital Amaurosis Caused by RPE65 Mutations Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4573 - 4577. [Abstract] [Full Text] [PDF]

				T. Sugita, M. Kondo, C.-H. Piao, Y. Ito, and H. Terasaki Correlation between Macular Volume and Focal Macular Electroretinogram in Patients with Retinitis Pigmentosa Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3551 - 3558. [Abstract] [Full Text] [PDF]

				M. D. Fischer, J. C. Fleischhauer, M. C. Gillies, F. K. Sutter, H. Helbig, and D. Barthelmes A New Method to Monitor Visual Field Defects Caused by Photoreceptor Degeneration by Quantitative Optical Coherence Tomography Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3617 - 3621. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, A. V. Cideciyan, T. S. Aleman, A. Sumaroka, A. J. Roman, L. M. Gardner, H. M. Prosser, M. Mishra, N. T. Bech-Hansen, W. Herrera, et al. Usher syndromes due to MYO7A, PCDH15, USH2A or GPR98 mutations share retinal disease mechanism Hum. Mol. Genet., August 1, 2008; 17(15): 2405 - 2415. [Abstract] [Full Text] [PDF]

				W. Herrera, T. S. Aleman, A. V. Cideciyan, A. J. Roman, E. Banin, T. Ben-Yosef, L. M. Gardner, A. Sumaroka, E. A. M. Windsor, S. B. Schwartz, et al. Retinal Disease in Usher Syndrome III Caused by Mutations in the Clarin-1 Gene Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2651 - 2660. [Abstract] [Full Text] [PDF]

				D. Barthelmes, M. C. Gillies, and F. K. P. Sutter Quantitative OCT Analysis of Idiopathic Perifoveal Telangiectasia Invest. Ophthalmol. Vis. Sci., May 1, 2008; 49(5): 2156 - 2162. [Abstract] [Full Text] [PDF]

				T. S. Aleman, A. V. Cideciyan, A. Sumaroka, E. A. M. Windsor, W. Herrera, D. A. White, S. Kaushal, A. Naidu, A. J. Roman, S. B. Schwartz, et al. Retinal Laminar Architecture in Human Retinitis Pigmentosa Caused by Rhodopsin Gene Mutations Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1580 - 1590. [Abstract] [Full Text] [PDF]

				C. Tappeiner, D. Barthelmes, M. H. Abegg, S. Wolf, and J. C. Fleischhauer Impact of Optic Media Opacities and Image Compression on Quantitative Analysis of Optical Coherence Tomography Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1609 - 1614. [Abstract] [Full Text] [PDF]

				A. Mataftsi, D. F. Schorderet, L. Chachoua, M. Boussalah, M. T. Nouri, D. Barthelmes, F.-X. Borruat, and F. L. Munier Novel TULP1 Mutation Causing Leber Congenital Amaurosis or Early Onset Retinal Degeneration Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5160 - 5167. [Abstract] [Full Text] [PDF]

				T. S. Aleman, A. V. Cideciyan, A. Sumaroka, S. B. Schwartz, A. J. Roman, E. A. M. Windsor, J. D. Steinberg, K. Branham, M. Othman, A. Swaroop, et al. Inner Retinal Abnormalities in X-linked Retinitis Pigmentosa with RPGR Mutations Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4759 - 4765. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, T. S. Aleman, A. V. Cideciyan, E. Heon, M. Golczak, W. A. Beltran, A. Sumaroka, S. B. Schwartz, A. J. Roman, E. A. M. Windsor, et al. Human cone photoreceptor dependence on RPE65 isomerase PNAS, September 18, 2007; 104(38): 15123 - 15128. [Abstract] [Full Text] [PDF]

				F. Simonelli, C. Ziviello, F. Testa, S. Rossi, E. Fazzi, P. E. Bianchi, M. Fossarello, S. Signorini, C. Bertone, S. Galantuomo, et al. Clinical and Molecular Genetics of Leber's Congenital Amaurosis: A Multicenter Study of Italian Patients Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4284 - 4290. [Abstract] [Full Text] [PDF]

				J. Chen and J. Nathans Genetic Ablation of Cone Photoreceptors Eliminates Retinal Folds in the Retinal Degeneration 7 (rd7) Mouse Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2799 - 2805. [Abstract] [Full Text] [PDF]

				S. G Jacobson, A. Sumaroka, T. S Aleman, A. V Cideciyan, M. Danciger, and D. B Farber Evidence for retinal remodelling in retinitis pigmentosa caused by PDE6B mutation Br. J. Ophthalmol., May 1, 2007; 91(5): 699 - 701. [Full Text] [PDF]

				S. G. Jacobson, A. V. Cideciyan, T. S. Aleman, A. Sumaroka, S. B. Schwartz, E. A. M. Windsor, A. J. Roman, E. Heon, E. M. Stone, and D. A. Thompson RDH12 and RPE65, Visual Cycle Genes Causing Leber Congenital Amaurosis, Differ in Disease Expression Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 332 - 338. [Abstract] [Full Text] [PDF]

				Y.-C. Hsu, J. J. Willoughby, A. K. Christensen, and A. M. Jensen Mosaic Eyes is a novel component of the Crumbs complex and negatively regulates photoreceptor apical size Development, December 15, 2006; 133(24): 4849 - 4859. [Abstract] [Full Text] [PDF]

				A. A. Azari, T. S. Aleman, A. V. Cideciyan, S. B. Schwartz, E. A. M. Windsor, A. Sumaroka, A. Y. Cheung, J. D. Steinberg, A. J. Roman, E. M. Stone, et al. Retinal Disease Expression in Bardet-Biedl Syndrome-1 (BBS1) Is a Spectrum from Maculopathy to Retina-Wide Degeneration Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5004 - 5010. [Abstract] [Full Text] [PDF]

				M. Richard, R. Roepman, W. M. Aartsen, A. G.S.H. van Rossum, A. I. den Hollander, E. Knust, J. Wijnholds, and F. P.M. Cremers Towards understanding CRUMBS function in retinal dystrophies Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R235 - R243. [Abstract] [Full Text] [PDF]

				S. Yzer, G. A. Fishman, J. Racine, S. Al-Zuhaibi, H. Chakor, A. Dorfman, J. Szlyk, P. Lachapelle, L. I. van den Born, R. Allikmets, et al. CRB1 Heterozygotes with Regional Retinal Dysfunction: Implications for Genetic Testing of Leber Congenital Amaurosis. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3736 - 3744. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, A. V. Cideciyan, A. Sumaroka, T. S. Aleman, S. B. Schwartz, E. A. M. Windsor, A. J. Roman, E. M. Stone, and I. M. MacDonald Remodeling of the Human Retina in Choroideremia: Rab Escort Protein 1 (REP-1) Mutations. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 4113 - 4120. [Abstract] [Full Text] [PDF]

				D. Barthelmes, F. K. Sutter, M. M. Kurz-Levin, M. M. Bosch, H. Helbig, G. Niemeyer, and J. C. Fleischhauer Quantitative Analysis of OCT Characteristics in Patients with Achromatopsia and Blue-Cone Monochromatism. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1161 - 1166. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, T. S. Aleman, A. V. Cideciyan, A. Sumaroka, S. B. Schwartz, E. A. M. Windsor, E. I. Traboulsi, E. Heon, S. J. Pittler, A. H. Milam, et al. Identifying photoreceptors in blind eyes caused by RPE65 mutations: Prerequisite for human gene therapy success PNAS, April 26, 2005; 102(17): 6177 - 6182. [Abstract] [Full Text] [PDF]

				A. V. Cideciyan, S. G. Jacobson, T. S. Aleman, D. Gu, S. E. Pearce-Kelling, A. Sumaroka, G. M. Acland, and G. D. Aguirre In vivo dynamics of retinal injury and repair in the rhodopsin mutant dog model of human retinitis pigmentosa PNAS, April 5, 2005; 102(14): 5233 - 5238. [Abstract] [Full Text] [PDF]

				S. B. Schwartz, T. S. Aleman, A. V. Cideciyan, E. A. M. Windsor, A. Sumaroka, A. J. Roman, T. Rane, E. E. Smilko, J. Bennett, E. M. Stone, et al. Disease Expression in Usher Syndrome Caused by VLGR1 Gene Mutation (USH2C) and Comparison with USH2A Phenotype Invest. Ophthalmol. Vis. Sci., February 1, 2005; 46(2): 734 - 743. [Abstract] [Full Text] [PDF]

				G. J. McKay, S. Clarke, J. A. Davis, D. A. C. Simpson, and G. Silvestri Pigmented Paravenous Chorioretinal Atrophy Is Associated with a Mutation within the Crumbs Homolog 1 (CRB1) Gene Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 322 - 328. [Abstract] [Full Text] [PDF]

				S. G. Jacobson, A. Sumaroka, T. S. Aleman, A. V. Cideciyan, S. B. Schwartz, A. J. Roman, R. R. McInnes, V. C. Sheffield, E. M. Stone, A. Swaroop, et al. Nuclear receptor NR2E3 gene mutations distort human retinal laminar architecture and cause an unusual degeneration Hum. Mol. Genet., September 1, 2004; 13(17): 1893 - 1902. [Abstract] [Full Text] [PDF]

				S. A. van de Pavert, A. Kantardzhieva, A. Malysheva, J. Meuleman, I. Versteeg, C. Levelt, J. Klooster, S. Geiger, M. W. Seeliger, P. Rashbass, et al. Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure J. Cell Sci., August 15, 2004; 117(18): 4169 - 4177. [Abstract] [Full Text] [PDF]

				T. S. Aleman, S. G. Jacobson, J. D. Chico, M. L. Scott, A. Y. Cheung, E. A. M. Windsor, M. Furushima, T. M. Redmond, J. Bennett, K. Palczewski, et al. Impairment of the Transient Pupillary Light Reflex in Rpe65-/- Mice and Humans with Leber Congenital Amaurosis Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1259 - 1271. [Abstract] [Full Text] [PDF]

				A. V. Cideciyan, T. S. Aleman, M. Swider, S. B. Schwartz, J. D. Steinberg, A. J. Brucker, A. M. Maguire, J. Bennett, E. M. Stone, and S. G. Jacobson Mutations in ABCA4 result in accumulation of lipofuscin before slowing of the retinoid cycle: a reappraisal of the human disease sequence Hum. Mol. Genet., March 1, 2004; 13(5): 525 - 534. [Abstract] [Full Text] [PDF]

				A. K. Mehalow, S. Kameya, R. S. Smith, N. L. Hawes, J. M. Denegre, J. A. Young, L. Bechtold, N. B. Haider, U. Tepass, J. R. Heckenlively, et al. CRB1 is essential for external limiting membrane integrity and photoreceptor morphogenesis in the mammalian retina Hum. Mol. Genet., September 1, 2003; 12(17): 2179 - 2189. [Abstract] [Full Text] [PDF]

				P. Castagnet, T. Mavlyutov, Y. Cai, F. Zhong, and P. Ferreira RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons Hum. Mol. Genet., August 1, 2003; 12(15): 1847 - 1863. [Abstract] [Full Text] [PDF]

This Article Abstract FREE Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Archive Download to citation manager Search for citing articles in: ISI Web of Science (56) Request Permissions Google Scholar Articles by Jacobson, S. G. Articles by Stone, E. M. Search for Related Content PubMed PubMed Citation Articles by Jacobson, S. G. Articles by Stone, E. M. Social Bookmarking          What's this?

Online ISSN 1460-2083 - Print ISSN 0964-6906

Copyright © 2009 Oxford University Press

Oxford Journals Oxford University Press

• Site Map
• Privacy Policy
• Frequently Asked Questions

Other Oxford University Press sites: Oxford University Press Oxford Journals China Oxford Journals Japan American National Biography Booksellers' Information Service Children's Fiction and Poetry Children's Reference Corporate & Special Sales Dictionaries Dictionary of National Biography Digital Reference English Language Teaching Higher Education Textbooks Humanities International Education Unit Law Medicine Music Online Products Oxford English Dictionary Reference Rights and Permissions Science School Books Social Sciences Very Short Introductions World's Classics