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© 1992 Oxford University Press

RESEARCH-ARTICLE

Cloning and characterization of an interstitial deletion at chromosome 11p15 in a sporadic breast cancer

Mutsuko Miyagi1,2, Johji Inazawa1, Ken-ichi Takita1 and Yusuke Nakamura1,*

1Department of Biochemistry, Cancer Institute 1-37-1, Kami-Ikebukuro, Toshima, Tokyo 170 2Second Department of Biochemistry, School of Medicine, University of the Ryukyus 207 Uehara, Nishihara-cho, Okinawa 903-01 Japan

* To whom correspondence should be addressed

Received August 28, 1992; Revised October 13, 1992; Accepted October 13, 1992

In a number of types of cancer including breast, hepatocellular, and bladder carcinoma, frequent losses of heterozygosity (LOH) on chromosome 11pl5 have indicated the presence of one or more tumor suppressor genes in this region. In the present study, we report the detection and characterization of a rearrangement at llpl5 in a sporadic breast carcinoma. 6 DNA clones encompassing the rearranged region were isolated; localization of both flanking clones to 11p15 by two-color fluorescent in situ hybridization (FISH) indicated that the rearrangement was caused by an interstitial deletion in the affected allele. Although it is uncertain whether the region between the flanking two loci was missing from tumor cells, our result implied that a putative tumor suppressor gene on chromosome 11p15 is located between the loci on either side of the interstitial deletion or may be interrupted by one of the breakpoints.


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