Human Molecular Genetics, 2001, Vol. 10, No. 19 2157-2164
© 2001 Oxford University Press
Genetic variation in the human urea transporter-2 is associated with variation in blood pressure
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA, 1Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, 2Section of General Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, 3Division of Cardiology, Department of Medicine, Taichung Veterans General Hospital, Taichung, Taiwan, 4Tri-Service General Hospital, Taipei, Taiwan, 5Division of Endocrinology, Stanford University School of Medicine, Stanford, CA 94305, USA, 6Department of Medicine, Brigham and Women’s Hospital, Boston, MA 02115, USA, 7Health Research and Policy, Stanford University School of Medicine, Stanford, CA 94305, USA and 8Hawaii Center for Health Research, 846 South Hotel Street, Suite 306, Honolulu, HI 9681, USA
The kidney, by regulating the volume of fluid in the body, plays a key role in regulating blood pressure (BP). The kidney uses primarily sodium and, to a lesser extent, urea to maintain the appropriate volume of fluid. Genetic variation in proteins that determine sodium reabsorption and excretion is known to significantly influence BP. However, the influence of genetic variation in urea transporters on BP has not been examined. We determined therefore whether nucleotide variation in the kidney-specific human urea transporter, HUT2, is associated with variation in BP. After determining the genomic structure of the coding sequence, seven single nucleotide polymorphisms (SNPs) were identified. Two of the SNPs result in Val/Ile and Ala/Thr amino acid substitutions at positions 227 and 357 in the HUT2 open reading frame, respectively. Another SNP is silent and four others are in introns or the 3' untranslated region. Over 1000 hypertensive and low-normotensive individuals of Chinese origin were typed for five of these SNPs using a high-throughput genotyping method. The Ile227 and Ala357 alleles were associated with low diastolic BP in men but not women, with odds ratios 2.1 [95% confidence interval (CI) 1.5–2.7, P < 0.001] and 1.5 (95% CI 1.2–1.8, P < 0.001), respectively. There was a similar trend for systolic BP, and odds ratios for the Ile227 and Ala357 alleles were 1.7 (95% CI 1.2–2.3, P = 0.002) and 1.3 (95% CI 1.1–1.6, P = 0.007), respectively, in men.
+ To whom correspondence should be addressed at: Bristol-Myers Squibb, Pharmaceutical Research Institute, PO Box 5400, Princeton, NJ 08543-5400, USA. Tel: +1 609 818 5342; Fax: +1 609 818 5839; Email: koustubh.ranade@bms.com
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